Biotin anti-Phosphotyrosine Antibody

Pricing & Availability
PY20 (See other available formats)
Regulatory Status
Other Names
Mouse IgG2b, κ
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Product Citations
Western blot analysis of Hela untreated (lane 1) cell and Hela treated with Pervanadate for 15min (lane 2) using Biotin-anti-phosphotyrosine antibody (PY20). GAPDH antibody (poly6314) was used as loading control.
  • PY20_phosphotyrosine_Biotin_WB_052814.jpg
    Western blot analysis of Hela untreated (lane 1) cell and Hela treated with Pervanadate for 15min (lane 2) using Biotin-anti-phosphotyrosine antibody (PY20). GAPDH antibody (poly6314) was used as loading control.
Cat # Size Price Quantity Avail. Save
309304 100 µg $185
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details
Technical data sheet

Product Details

Human, Mouse, Rat, All Species
Antibody Type
Host Species
KLH-conjugated phosphotyrosine
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 5 µg/5ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
  2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
  3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
  4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
  5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
  6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
Product Citations
  1. Mukherjee K, et al. 2020. Front Immunol. 11:332. PubMed
AB_314766 (BioLegend Cat. No. 309304)

Antigen Details



Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
5800 View all products for this Gene ID
View information about Phosphotyrosine on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 12/10/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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