Biotin anti-Phosphotyrosine Antibody

Pricing & Availability
Clone
PY20 (See other available formats)
Other Names
p-Tyr
Isotype
Mouse IgG2b, κ
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Product Citations
publications
PY20_phosphotyrosine_Biotin_WB_052814.jpg
Western blot analysis of Hela untreated (lane 1) cell and Hela treated with Pervanadate for 15min (lane 2) using Biotin-anti-phosphotyrosine antibody (PY20). GAPDH antibody (poly6314) was used as loading control.
  • PY20_phosphotyrosine_Biotin_WB_052814.jpg
    Western blot analysis of Hela untreated (lane 1) cell and Hela treated with Pervanadate for 15min (lane 2) using Biotin-anti-phosphotyrosine antibody (PY20). GAPDH antibody (poly6314) was used as loading control.
Cat # Size Price Quantity Avail. Save
309304 100 µg $185
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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated phosphotyrosine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
ICFC, IF, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 5 µg/5ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
  2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
  3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
  4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
  5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
  6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
RRID
AB_314766 (BioLegend Cat. No. 309304)

Antigen Details

Distribution

Phospho-Specific

Function
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
5800 View all products for this Gene ID
UniProt
View information about Phosphotyrosine on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 05/28/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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