APC anti-Phosphotyrosine Antibody

Pricing & Availability
Clone
PY20 (See other available formats)
Other Names
p-Tyr
Isotype
Mouse IgG2b, κ
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Product Citations
publications
PY20_APC_Phosphotyrosine_Antibody_032116
Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H2O2 (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) APC.
  • PY20_APC_Phosphotyrosine_Antibody_032116
    Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H2O2 (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) APC.
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Cat # Size Price Quantity Avail. Save
309312 100 µg $225
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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated phosphotyrosine
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
  2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
  3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
  4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
  5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
  6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
RRID
AB_2572197 (BioLegend Cat. No. 309312)

Antigen Details

Distribution

Phospho-Specific

Function
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID
5800 View all products for this Gene ID
UniProt
View information about Phosphotyrosine on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/24/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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