- PY20 (See other available formats)
- Other Names
- Mouse IgG2b, κ
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- Product Citations
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.Product Details
- Human, Mouse, Rat, All Species
- Antibody Type
- Host Species
- KLH-conjugated phosphotyrosine
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
IF - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use dilution buffer containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.
(PubMed link indicates BioLegend citation)
- Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
- Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
- Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
- Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
- Product Citations
AB_528951 (BioLegend Cat. No. 309308)
- Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Gene ID
- 5800 View all products for this Gene ID
- View information about Phosphotyrosine on UniProt.org
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