- PY20 (See other available formats)
- Other Names
- Mouse IgG2b, κ
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- Product Citations
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.Product Details
- Human, Mouse, Rat, All Species
- Antibody Type
- Host Species
- KLH-conjugated phosphotyrosine
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored at -20°C.
WB - Quality tested
ICC - Validated
ICFC, IP- Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 0.125 - 1.0 µg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.
(PubMed link indicates BioLegend citation)
- Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
- Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
- Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (ICC)
- Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
- Product Citations
AB_314763 (BioLegend Cat. No. 309301)
AB_314764 (BioLegend Cat. No. 309302)
- Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Gene ID
- 5800 View all products for this Gene ID
- View information about Phosphotyrosine on UniProt.org
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