- Clone
- RA3-6B2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B220
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

-
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488 -
C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 594 (red), 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 647 (blue), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective. -
Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD3ε Antibody (clone 145-2C11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
Watch the video.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
103228 | 25 µg | £70 | ||||
103225 | 100 µg | £159 |
CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Reported Reactivity
- Cat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Abelson murine leukemia virus-induced pre-B tumor cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F, 3D IHC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
-
Application References
(PubMed link indicates BioLegend citation) -
- Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
- George A, et al. 1994. J. Immunol. 152:1014. (Activ)
- Asensi V, et al. 1989. Immunology 68:204. (Activ)
- Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
- Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
- Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
- Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
- Chang C L-T, et al. 2007. J. Immunol. 178:6984.
- Fazilleau N, et al. 2007. Nature Immunol. 8:753.
- Lang GL, et al. 2008. Blood 111:2158. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_492874 (BioLegend Cat. No. 103228)
AB_389308 (BioLegend Cat. No. 103225)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
B cells, T cell subset, NK cell subset
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851. - Gene ID
- 19264 View all products for this Gene ID 5788 View all products for this Gene ID
- UniProt
- View information about CD45R on UniProt.org
Related FAQs
Other Formats
View All CD45R/B220 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Alexa Fluor® 594 anti-mouse/human CD45R/B220
-
APC anti-mouse/human CD45R/B220
-
Biotin anti-mouse/human CD45R/B220
-
FITC anti-mouse/human CD45R/B220
-
PE anti-mouse/human CD45R/B220
-
PE/Cyanine5 anti-mouse/human CD45R/B220
-
Purified anti-mouse/human CD45R/B220
-
PE/Cyanine7 anti-mouse/human CD45R/B220
-
APC/Cyanine7 anti-mouse/human CD45R/B220
-
Alexa Fluor® 488 anti-mouse/human CD45R/B220
-
Alexa Fluor® 647 anti-mouse/human CD45R/B220
-
Pacific Blue™ anti-mouse/human CD45R/B220
-
Alexa Fluor® 700 anti-mouse/human CD45R/B220
-
PerCP anti-mouse/human CD45R/B220
-
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220
-
Brilliant Violet 421™ anti-mouse/human CD45R/B220
-
Brilliant Violet 570™ anti-mouse/human CD45R/B220
-
Brilliant Violet 650™ anti-mouse/human CD45R/B220
-
Brilliant Violet 605™ anti-mouse/human CD45R/B220
-
Brilliant Violet 785™ anti-mouse/human CD45R/B220
-
Brilliant Violet 510™ anti-mouse/human CD45R/B220
-
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)
-
Brilliant Violet 711™ anti-mouse/human CD45R/B220
-
PE/Dazzle™ 594 anti-mouse/human CD45R/B220
-
APC/Fire™ 750 anti-mouse/human CD45R/B220
-
Brilliant Violet 750™ anti-mouse/human CD45R/B220
-
TotalSeq™-A0103 anti-mouse/human CD45R/B220
-
Spark Blue™ 550 anti-mouse/human CD45R/B220
-
Spark NIR™ 685 anti-mouse/human CD45R/B220
-
TotalSeq™-B0103 anti-mouse/human CD45R/B220
-
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220
-
TotalSeq™-C0103 anti-mouse/human CD45R/B220
-
PE/Fire™ 640 anti-mouse/human CD45R/B220
-
APC/Fire™ 810 anti-mouse/human CD45R/B220
-
PE/Fire™ 700 anti-mouse/human CD45R/B220
-
Spark Violet™ 538 anti-mouse/human CD45R/B220
-
Spark YG™ 581 anti-mouse/human CD45R/B220
-
Spark YG™ 570 anti-mouse/human CD45R/B220
-
PE/Fire™ 810 anti-mouse/human CD45R/B220
-
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody
-
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody
-
Spark Red™ 718 anti-mouse/human CD45R/B220
Follow Us