Alexa Fluor® 488 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
RA3-6B2 (See other available formats)
Regulatory Status
Other Names
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 1_RA3-6B2_AF488_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 2_RA3-6B2_A488_B220_Antibody_IHC-F_080519
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 594 (red), 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 647 (blue), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3_RA3-6B2_A488_CD45R-B220_Antibody_3_092021.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD3ε Antibody (clone 145-2C11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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103228 25 µg £70
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103225 100 µg £159
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CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Antibody Type
Host Species
Abelson murine leukemia virus-induced pre-B tumor cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  3. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  4. Kitai H, et al. 2022. BMC Biol. 20:248. PubMed
  5. Mostofa A, et al. 2021. JCI Insight. 6:. PubMed
  6. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  7. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  8. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  9. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  10. Schnoor M, et al. 2020. Bio Protoc. 10:e3842. PubMed
  11. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  12. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  13. Serger E, et al. 2022. Nature. 607:585. PubMed
  14. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  15. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  16. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  17. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  18. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  19. Luo Z, et al. 2013. J Control Release. 170:259. PubMed
  20. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  21. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  22. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  23. Ge S, et al. 2012. Nephrol Dial Transplant. 209:35:00. PubMed
  24. Wang C, et al. 2014. Vaccine. 32:5475. PubMed
  25. Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed
  26. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  27. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  28. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  29. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  30. Li N, et al. 2021. iScience. 24:103163. PubMed
  31. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  32. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  33. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  34. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  35. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  36. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  37. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  38. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  39. Werner A, et al. 2021. iScience. 24:103076. PubMed
  40. Chen B, et al. 2021. Cell. 184:6262. PubMed
  41. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  42. Nunes NS, et al. 2019. EBioMedicine. 45:495. PubMed
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  44. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
AB_492874 (BioLegend Cat. No. 103228)
AB_389308 (BioLegend Cat. No. 103225)

Antigen Details

Protein tyrosine phosphatase (PTP) family, 180-240 kD

B cells, T cell subset, NK cell subset

Phosphatase, T and B cell activation
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
View information about CD45R on

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Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV™ 395 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 6    Revision Date: 09/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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