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Alexa Fluor® 488 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_RA3-6B2_AF488_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 1_RA3-6B2_AF488_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 488
  • 2_RA3-6B2_A488_B220_Antibody_IHC-F_080519
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 594 (red), 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 647 (blue), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3_RA3-6B2_A488_CD45R-B220_Antibody_3_092021.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD3ε Antibody (clone 145-2C11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Compare all formats See Alexa Fluor® 488 spectral data
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103228 25 µg £70
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103225 100 µg £159
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 μg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  2. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  3. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  4. Kitai H, et al. 2022. BMC Biol. 20:248. PubMed
  5. Mostofa A, et al. 2021. JCI Insight. 6:. PubMed
  6. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  7. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  8. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  9. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  10. Schnoor M, et al. 2020. Bio Protoc. 10:e3842. PubMed
  11. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  12. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  13. Serger E, et al. 2022. Nature. 607:585. PubMed
  14. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  15. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  16. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  17. Lin J, Sorger M 2015. Sci Rep. 6: 8390. PubMed
  18. Luo Z, et al. 2013. J Control Release. 170:259. PubMed
  19. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  20. Ge S, et al. 2012. Nephrol Dial Transplant. 209:35:00. PubMed
  21. Wang C, et al. 2014. Vaccine. 32:5475. PubMed
  22. Frasch S, et al. 2016. J Immunol. 197: 1425 - 1434. PubMed
  23. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  24. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  25. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  26. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  27. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  28. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  29. Tsai F, et al. 2017. J Exp Med. 214:3753. PubMed
  30. Li N, et al. 2021. iScience. 24:103163. PubMed
  31. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  32. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  33. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  34. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  35. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  36. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  37. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  38. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  39. Werner A, et al. 2021. iScience. 24:103076. PubMed
  40. Chen B, et al. 2021. Cell. 184:6262. PubMed
  41. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
  42. Nunes NS, et al. 2019. EBioMedicine. 45:495. PubMed
  43. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  44. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_492874 (BioLegend Cat. No. 103228)
AB_389308 (BioLegend Cat. No. 103225)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 6    Revision Date: 09/20/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 594 anti-mouse/human CD45R/B220

  • APC anti-mouse/human CD45R/B220

  • Biotin anti-mouse/human CD45R/B220

  • FITC anti-mouse/human CD45R/B220

  • PE anti-mouse/human CD45R/B220

  • PE/Cyanine5 anti-mouse/human CD45R/B220

  • Purified anti-mouse/human CD45R/B220

  • PE/Cyanine7 anti-mouse/human CD45R/B220

  • APC/Cyanine7 anti-mouse/human CD45R/B220

  • Alexa Fluor® 488 anti-mouse/human CD45R/B220

  • Alexa Fluor® 647 anti-mouse/human CD45R/B220

  • Pacific Blue™ anti-mouse/human CD45R/B220

  • Alexa Fluor® 700 anti-mouse/human CD45R/B220

  • PerCP anti-mouse/human CD45R/B220

  • PerCP/Cyanine5.5 anti-mouse/human CD45R/B220

  • Brilliant Violet 421™ anti-mouse/human CD45R/B220

  • Brilliant Violet 570™ anti-mouse/human CD45R/B220

  • Brilliant Violet 650™ anti-mouse/human CD45R/B220

  • Brilliant Violet 605™ anti-mouse/human CD45R/B220

  • Brilliant Violet 785™ anti-mouse/human CD45R/B220

  • Brilliant Violet 510™ anti-mouse/human CD45R/B220

  • Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)

  • Brilliant Violet 711™ anti-mouse/human CD45R/B220

  • PE/Dazzle™ 594 anti-mouse/human CD45R/B220

  • APC/Fire™ 750 anti-mouse/human CD45R/B220

  • Brilliant Violet 750™ anti-mouse/human CD45R/B220

  • TotalSeq™-A0103 anti-mouse/human CD45R/B220

  • Spark Blue™ 550 anti-mouse/human CD45R/B220

  • Spark NIR™ 685 anti-mouse/human CD45R/B220

  • TotalSeq™-B0103 anti-mouse/human CD45R/B220

  • Ultra-LEAF™ Purified anti-mouse/human CD45R/B220

  • TotalSeq™-C0103 anti-mouse/human CD45R/B220

  • PE/Fire™ 640 anti-mouse/human CD45R/B220

  • APC/Fire™ 810 anti-mouse/human CD45R/B220

  • PE/Fire™ 700 anti-mouse/human CD45R/B220

  • Spark Violet™ 538 anti-mouse/human CD45R/B220

  • Spark YG™ 581 anti-mouse/human CD45R/B220

  • Spark YG™ 570 anti-mouse/human CD45R/B220

  • PE/Fire™ 810 anti-mouse/human CD45R/B220

  • Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody

  • Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody

  • Spark Red™ 718 anti-mouse/human CD45R/B220
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