APC/Cyanine7 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RA3-6B2_APCCy7_060308
C57BL/6 splenocytes stained with RA3-6B2 APC/Cyanine7
  • RA3-6B2_APCCy7_060308
    C57BL/6 splenocytes stained with RA3-6B2 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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103223 25 µg £61
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103224 100 µg £135
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
  5. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  6. Huang Y, et al. 2015. Proc Natl Acad Sci U S A. 112:39. PubMed
  7. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  8. Ishikura S, et al. 2016. J Biol Chem. 291: 15282 - 15291. PubMed
  9. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  10. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  11. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  12. Lechuga-Vieco AV, et al. 2020. Sci Adv. 6:eaba5345. PubMed
  13. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  14. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  15. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  16. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  17. Chen S, et al. 2022. Cancer Discov. :. PubMed
  18. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  19. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  20. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  21. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  22. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  23. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  24. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  25. Jacoby E, et al. 2016. Blood. 127: 1361 - 1370. PubMed
  26. D'Auria K, et al. 2013. Infect Immun . 81:3814. PubMed
  27. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  28. Shinoda K, et al. 2020. Cell Reports. 29(13):4471-4481.e6.. PubMed
  29. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  30. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  31. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  32. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  33. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  34. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  35. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  36. Thayer T, et al. 2011. Diabetes. 60:2144. PubMed
  37. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  38. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  39. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  40. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  41. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  42. Dema B, et al. 2014. J Exp Med. 211:2159. PubMed
  43. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  44. Lam WY et al. 2018. Cell reports. 24(9):2479-2492 . PubMed
  45. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  46. Muppidi J, et al. 2015. J Exp Med. 212: 2213 - 2222. PubMed
  47. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  48. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  49. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  50. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  51. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
  52. Rivas MA, et al. 2021. Front Immunol. 12:688493. PubMed
  53. Sato R, et al. 2020. Int Immunol. 499:32. PubMed
  54. Benet Z, et al. 2021. Cell Reports. 34(6):108733. PubMed
  55. Yao M, et al. 2020. Cell. 180(3):502-520. PubMed
  56. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  57. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  58. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  59. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  60. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  61. Sullivan JA, et al. 2020. Cell Rep. 30:1039. PubMed
  62. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  63. Roy K, et al. 2019. Immunity. 50:616. PubMed
  64. Bei T, et al. 2021. Molecules. 26:. PubMed
  65. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  66. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  67. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  68. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  69. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  70. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  71. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  72. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  73. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  74. Jacoby E, et al. 2016. Nat Commun. 7:12320. PubMed
  75. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
  76. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  77. Gough D, et al. 2014. Blood. 124:2252. PubMed
  78. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  79. Huang Y, et al. 2016. J Immunol. 196: 217 - 231. PubMed
  80. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  81. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  82. Xiao G, et al. 2018. Cell. 173:470. PubMed
  83. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  84. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  85. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  86. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  87. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  88. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  89. Yan-Feng L, et al. 2017. Sci Rep. 7:40133. PubMed
  90. Uribe-Herranz M, et al. 2018. JCI Insight. 3. PubMed
  91. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  92. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  93. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  94. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  95. Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
  96. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  97. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  98. Kim SK, et al. 2018. Genes Dev. 32:849. PubMed
  99. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  100. Lee HY, et al. 2021. Curr Protoc. 1:e272. PubMed
  101. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  102. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  103. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  104. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  105. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  106. Altin J, et al. 2014. Proc Natl Acad Sci U S A. 111:2067. PubMed
  107. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  108. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  109. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  110. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  111. Sato-Hashimoto M, et al. 2011. J Immunol. 187:291. PubMed
  112. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
  113. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  114. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  115. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
RRID
AB_313006 (BioLegend Cat. No. 103223)
AB_313007 (BioLegend Cat. No. 103224)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 3    Revision Date: 04/25/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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