- RA3-6B2 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.Product Details
- Verified Reactivity
- Mouse, Human
- Reported Reactivity
- Antibody Type
- Host Species
- Abelson murine leukemia virus-induced pre-B tumor cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-F - Quality tested
FC, 3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2.5-5 μg/mL is recommended. For immunofluorescence microscopy, a concentration range of 1.25 - 10 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.
(PubMed link indicates BioLegend citation)
- Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
- George A, et al. 1994. J. Immunol. 152:1014. (Activ)
- Asensi V, et al. 1989. Immunology 68:204. (Activ)
- Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
- Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
- Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
- Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
- Chang C L-T, et al. 2007. J. Immunol. 178:6984.
- Fazilleau N, et al. 2007. Nature Immunol. 8:753.
- Lang GL, et al. 2008. Blood 111:2158. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2563229 (BioLegend Cat. No. 103254)
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
B cells, T cell subset, NK cell subset
- Phosphatase, T and B cell activation
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 19264 View all products for this Gene ID 5788 View all products for this Gene ID
- View information about CD45R on UniProt.org
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Alexa Fluor® 594 anti-mouse/human CD45R/B220
APC anti-mouse/human CD45R/B220
Biotin anti-mouse/human CD45R/B220
FITC anti-mouse/human CD45R/B220
PE anti-mouse/human CD45R/B220
PE/Cyanine5 anti-mouse/human CD45R/B220
Purified anti-mouse/human CD45R/B220
PE/Cyanine7 anti-mouse/human CD45R/B220
APC/Cyanine7 anti-mouse/human CD45R/B220
Alexa Fluor® 488 anti-mouse/human CD45R/B220
Alexa Fluor® 647 anti-mouse/human CD45R/B220
Pacific Blue™ anti-mouse/human CD45R/B220
Alexa Fluor® 700 anti-mouse/human CD45R/B220
PerCP anti-mouse/human CD45R/B220
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220
Brilliant Violet 421™ anti-mouse/human CD45R/B220
Brilliant Violet 570™ anti-mouse/human CD45R/B220
Brilliant Violet 650™ anti-mouse/human CD45R/B220
Brilliant Violet 605™ anti-mouse/human CD45R/B220
Brilliant Violet 785™ anti-mouse/human CD45R/B220
Brilliant Violet 510™ anti-mouse/human CD45R/B220
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)
Brilliant Violet 711™ anti-mouse/human CD45R/B220
PE/Dazzle™ 594 anti-mouse/human CD45R/B220
APC/Fire™ 750 anti-mouse/human CD45R/B220
Brilliant Violet 750™ anti-mouse/human CD45R/B220
TotalSeq™-A0103 anti-mouse/human CD45R/B220
Spark Blue™ 550 anti-mouse/human CD45R/B220
Spark NIR™ 685 anti-mouse/human CD45R/B220
TotalSeq™-B0103 anti-mouse/human CD45R/B220
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220
TotalSeq™-C0103 anti-mouse/human CD45R/B220
PE/Fire™ 640 anti-mouse/human CD45R/B220
APC/Fire™ 810 anti-mouse/human CD45R/B220
PE/Fire™ 700 anti-mouse/human CD45R/B220
Spark Violet™ 538 anti-mouse/human CD45R/B220
Spark YG™ 581 anti-mouse/human CD45R/B220
Spark YG™ 570 anti-mouse/human CD45R/B220
PE/Fire™ 810 anti-mouse/human CD45R/B220
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody
Spark Red™ 718 anti-mouse/human CD45R/B220