PE anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RA3-6B2_PE_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 PE
  • RA3-6B2_PE_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 PE
  • RA3-6B2_PE_CD45RB220_Antibody__04292022
    C57BL/6 mouse splenocytes were stained with CD45R/B220 (clone RA3-6B2) PE (solid line) or rat IgG2a, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
103207 50 µg £31
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103208 200 µg £74
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bermea KC, et al. 2022. J Vis Exp. . PubMed
  2. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  3. Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed
  4. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed
  6. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  7. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  8. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  9. Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed
  10. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  11. Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed
  12. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  13. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  14. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  15. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  16. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  17. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  18. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  19. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  20. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  21. Li Y, et al. 2016. Nat Commun. 7:10836. PubMed
  22. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  23. Ma Z, et al. 2016. Clin Cancer Res. 22: 2969 - 2980. PubMed
  24. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  25. Franks SE, et al. 2019. J Immunol. 202:3381. PubMed
  26. Tan X, et al. 2021. Front Oncol. 11:768222. PubMed
  27. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  28. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  29. Zape J, et al. 2017. Cell Cycle. 10.1080/15384101.2017.1361569. PubMed
  30. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  31. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  32. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  33. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  34. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  35. Nguyen T, et al. 2014. J Exp Med. 211:1011. PubMed
  36. Cravens P, et al. 2016. PLoS One. 11:e0165248. PubMed
  37. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  38. Zhang J, et al. 2022. Front Immunol. 13:931740. PubMed
  39. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  40. Artinger K, et al. 2015. PLoS One. 10: 0135087. PubMed
  41. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  42. Li J, et al. 2019. JCI Insight. 5. PubMed
  43. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  44. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  45. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  46. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  47. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  48. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  49. Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed
  50. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  51. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  52. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  53. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  54. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  55. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  56. Khatib–Massalha E, et al. 2020. Nat Commun. 2.921527778. PubMed
  57. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  58. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  59. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  60. Nguyen T, et al. 2016. Sci Rep. 6:36906. PubMed
  61. Fu T, et al. 2011. Mol Immunol. 48:751. PubMed
  62. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  63. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  64. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  65. Cho K, et al. 2021. iScience. 24:103117. PubMed
  66. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  67. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  68. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  69. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  70. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  71. White C, et al. 2015. J Immunol. 194:697. PubMed
  72. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  73. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  74. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  75. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  76. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  77. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  78. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  79. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  80. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  81. Wu T, et al. 2014. J Leukoc Biol. 95:215. PubMed
  82. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  83. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  84. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  85. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  86. Wang D, et al. 2005. Int Immunol. 1.527083333. PubMed
  87. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  88. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  89. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  90. Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed
  91. Liang Q, et al. 2020. Front Microbiol. 11:571847. PubMed
  92. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  93. Lu W, et al. 2016. PLoS One. 11: 0149543. PubMed
  94. Faingold O, et al. 2013. J Biol Chem. 288:32852. PubMed
  95. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  96. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  97. Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
  98. Zhu L, et al. 2022. Cells. 11:. PubMed
  99. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  100. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  101. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  102. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  103. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  104. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  105. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  106. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  107. Lim J, et al. 2017. Cell. 169:523. PubMed
  108. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  109. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  110. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  111. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  112. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  113. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
  114. Chang C, et al. 2007. J Immunol . 178:6984. PubMed
  115. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  116. Oh B, et al. 2017. Plast Reconstr Surg Glob Open. 5:e1595. PubMed
  117. Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed
  118. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  119. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  120. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  121. Uotila LM, et al. 2017. J Immunol. 199:3644. PubMed
  122. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  123. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  124. Gawish R, et al. 2022. Elife. 11:. PubMed
  125. Feng M, et al. 2012. Int Immunopharmacol. 13:135. PubMed
  126. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  127. Madsen P, et al. 2016. J Neurosci. 36: 5128 - 5143. PubMed
  128. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  129. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
  130. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  131. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  132. Zheng QY, et al. 2019. Front Immunol. 10:1866. PubMed
  133. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  134. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
RRID
AB_312992 (BioLegend Cat. No. 103207)
AB_312993 (BioLegend Cat. No. 103208)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV™ 395 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04/25/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account