Alexa Fluor 700 anti-mouse/human CD45R/B220 Antibody anti-CD45R

Pricing & Availability

Clone: RA3-6B2 (See other available formats)
Regulatory Status: RUO
Other Names: B220
Isotype: Rat IgG2a, κ
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Product Citations: publications

C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700

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Cat #

Size

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103231

25 µg

£65

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103232

100 µg

£145

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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cat

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.

Application References (PubMed link indicates BioLegend citation)

Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
George A, et al. 1994. J. Immunol. 152:1014. (Activ)
Asensi V, et al. 1989. Immunology 68:204. (Activ)
Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Chang C L-T, et al. 2007. J. Immunol. 178:6984.
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Lang GL, et al. 2008. Blood 111:2158. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
Yamin R, et al. 2021. Nature. 599:465. PubMed
Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
Yang X, et al. 2020. Blood Sci. 2:89. PubMed
Shang Y, et al. 2016. Nat Immunol. 10.1038/ni.3486. PubMed
Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
Perry JSA, et al. 2018. Immunity. 48:923. PubMed
Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
Agrawal M, et al. 2021. Cells. 10:. PubMed
Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
Chauveau A, et al. 2020. Immunity. 52:794. PubMed
Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
Panea C, et al. 2021. Commun Biol. 4:913. PubMed
La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
Spix B, et al. 2022. Nat Commun. 13:318. PubMed
Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
Lee S, et al. 2021. Autophagy. 18:1062. PubMed

RRID

AB_493716 (BioLegend Cat. No. 103231)
AB_493717 (BioLegend Cat. No. 103232)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt: View information about CD45R on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

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Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220	RA3-6B2	FC
Biotin anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
FITC anti-mouse/human CD45R/B220	RA3-6B2	FC
PE anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Cyanine5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)	RA3-6B2	FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 750 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Spark Blue™ 550 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark NIR™ 685 anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
PE/Fire™ 640 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Fire™ 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Violet™ 538 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 581 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 570 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Red™ 718 anti-mouse/human CD45R/B220	RA3-6B2	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 06/27/2014

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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