Alexa Fluor® 647 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
1-17A2_Alx647_021606
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 647
  • 1-17A2_Alx647_021606
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 647
  • 2-17A2_A647_CD3_Antibody_2_121018
    Dissected C57/B6 mouse spleen was immersed in 4% paraformaldehyde (PFA) overnight followed by 30% sucrose immersion overnight and frozen in OCT. Frozen section was blocked with 5% FBS and 5% mouse serum for 30 minutes at room temperature. Then the tissue section was stained with 5 µg/mL of anti-mouse CD3 (clone 17A2) Alexa Fluor® 647 (red) and 5 µg/mL of anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 594 (blue) and 5 µg/mL of anti-mouse CD169 (clone 3D6-112) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
  • 3-17A2_A647_CD3_Antibody_3_050219
    Formalin-fixed, 400 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD21/35 (CR2/CR1)(clone 7E9) Alexa Fluor® 594 (red), CD169 (Siglec-1) (clone 3D6.112) Alexa Fluor® 488 (green), and CD3 (clone 17A2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 235 µm imaging depth on a confocal microscope. Scale bar: 100 µm. Watch the video.
  • 4-17A2_A647_CD3_Antibody_4_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD37 Antibody (clone Duno85) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
See Alexa Fluor® 647 spectral data
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100209 100 µg £133
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Agudo J et al. 2018. Immunity. 48(2):271-285 . PubMed
  2. Crinier A, et al. 2018. Immunity. 49:971. PubMed
  3. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  4. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  5. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  6. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  7. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  8. Bárcena C et al. 2019. EBioMedicine. 43:513-524 . PubMed
  9. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  10. Ardehali R, et al. 2013. Proc Natl Acad Sci U S A. 110:3405. PubMed
  11. Xiong S 2013. J Immunol. 190:3267. PubMed
  12. Hiraoka N, et al. 2011. Gastroenterology. 140:310. PubMed
  13. Sluis T, et al. 2015. Clin Cancer Res. 21:781. PubMed
  14. Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed
  15. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  16. Chang H, et al. 2016. J Immunol. 197: 2473 - 2484. PubMed
  17. Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed
  18. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  19. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  20. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  21. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
RRID
AB_389323 (BioLegend Cat. No. 100209)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 09/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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