FITC anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1
LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
  • 16-10A1
    LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
104705 50 µg £41.00
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104706 500 µg £127.00
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Cross-Reactivity: Dog (Canine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  2. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  3. Porciello N, et al. 2018. Nat Commun. 9:1080. PubMed
  4. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  5. Jiang H, et al. 2018. Front Pharmacol. 9:612. PubMed
  6. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  7. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  8. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  9. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  10. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  11. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  12. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  13. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  14. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  15. Ji W, et al. 2018. Evid Based Complement Alternat Med. 2017:3272649. PubMed
  16. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  17. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  18. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  19. Li X, et al. 2009. PLoS One. 4:e6385. PubMed
  20. Kis-Toth K, et al. 2011. J Immunol. 187:1273. PubMed
  21. Gravano D, et al. 2011. PLoS One. 6:e13517. PubMed
  22. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  23. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  24. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  25. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  26. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  27. Balashov L 2015. Mult Scler. 21: 1131-1138. PubMed
  28. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  29. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  30. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  31. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  32. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  33. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
RRID
AB_313126 (BioLegend Cat. No. 104705)
AB_313127 (BioLegend Cat. No. 104706)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/18/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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