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PE anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_PE_032309.jpg
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
  • 16-10A1_PE_032309.jpg
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
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104707 50 µg £76
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104708 200 µg £201
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Sohn HS, et al. 2022. Sci Adv. 8:eabo5284. PubMed
  2. Chen H, et al. 2023. Nat Commun. 14:941. PubMed
  3. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  4. Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed
  5. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  6. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  7. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  8. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  9. Li Y, et al. 2023. Front Cell Infect Microbiol. 13:1117230. PubMed
  10. Wu N, et al. 2023. Front Bioeng Biotechnol. 11:1173381. PubMed
  11. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  12. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  13. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  14. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  15. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  16. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  17. Sun Y, et al. 2023. Bioact Mater. 22:47. PubMed
  18. Liu M, et al. 2022. Cells. 11: . PubMed
  19. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  20. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  21. Han J, et al. 2023. Front Microbiol. 13:1067725. PubMed
  22. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  23. Rozanski C, et al. 2011. J Exp Med. 208:1435. PubMed
  24. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  25. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  26. Wilhelm J, et al. 2021. J Control Release. 329:353. PubMed
  27. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  28. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  29. Wei H, et al. 2021. Malar J. 20:89. PubMed
  30. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  31. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  32. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  33. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  34. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  35. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  36. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  37. Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
  38. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  39. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  40. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  41. Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
  42. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  43. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  44. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  45. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  46. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  47. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  48. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  49. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  50. Zhang F, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.019. PubMed
  51. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  52. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  53. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  54. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  55. Xie Z, et al. 2018. J Am Heart Assoc. 7:e007442. PubMed
  56. Florêncio KGD, et al. 2022. Front Immunol. 13:941757. PubMed
  57. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  58. Guo L, et al. 2021. Vaccines (Basel). 9:. PubMed
  59. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  60. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  61. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  62. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  63. Matundan H, et al. 2019. J Virol. 93. PubMed
  64. Matundan HH, et al. 2021. J Virol. 95:e0103621. PubMed
  65. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  66. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  67. Vogel A, et al. 2022. STAR Protoc. 3:101653. PubMed
  68. Wong E, et al. 2012. J Immunol. 189:5667. PubMed
  69. White C, et al. 2015. J Immunol. 194:697. PubMed
  70. Yang C, et al. 2019. Nanomedicine (Lond). 14:2423. PubMed
  71. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  72. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  73. Zhang X, et al. 2022. JCI Insight. 7:. PubMed
  74. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  75. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  76. Marshall N, et al. 2012. Cancer Res. 72:581. PubMed
  77. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  78. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  79. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  80. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  81. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  82. Kelly SH, et al. 2021. ACS Biomater Sci Eng. 7:1876. PubMed
  83. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  84. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  85. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  86. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  87. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  88. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  89. Qi J, et al. 2021. Nat Commun. 12:4755. PubMed
  90. Li J, et al. 2020. Development. :. PubMed
  91. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  92. Simmons D, et al. 2012. J Immunol. 188:3116. PubMed
  93. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
  94. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  95. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  96. Feng S, et al. 2021. Exp Ther Med. 21:20. PubMed
  97. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  98. Piñón-Zárate G, et al. 2022. Molecules. 27:. PubMed
  99. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  100. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
  101. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  102. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  103. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  104. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  105. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  106. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  107. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  108. Kadir NA, et al. 2020. Pathogens. 9:00. PubMed
  109. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  110. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  111. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  112. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  113. Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed
  114. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  115. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  116. Zhang Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  117. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
  118. Jeisy-Scott V, et al. 2012. J Virol. 86:10988. PubMed
RRID
AB_313128 (BioLegend Cat. No. 104707)
AB_313128 (BioLegend Cat. No. 104708)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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