Loading...

Biotin anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
16-10A1_Biotin_CD80_Antibody_FC_090414.jpg
LPS-stimulated (3 days) BALB/c splenocytes were stained with biotinylated CD80 (clone 16-10A1, filled histogram) or Armenian hamster IgG (HTK888) isotype control (open histrogram), followed by Sav-PE.
  • 16-10A1_Biotin_CD80_Antibody_FC_090414.jpg
    LPS-stimulated (3 days) BALB/c splenocytes were stained with biotinylated CD80 (clone 16-10A1, filled histogram) or Armenian hamster IgG (HTK888) isotype control (open histrogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability Save
104703 50 µg £53
Check Availability


Need larger quantities of this item?
Request Bulk Quote
104704 500 µg £180
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  2. Peraino J, et al. 2013. J Immunol Methods. 31:391. PubMed
  3. Aichinger M, et al. 2013. J Exp Med. 210:287. PubMed
  4. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  5. Garidou L, et al. 2009. J Virol. 83:8905. PubMed
RRID
AB_313124 (BioLegend Cat. No. 104703)
AB_313125 (BioLegend Cat. No. 104704)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD80

  • FITC anti-mouse CD80

  • PE anti-mouse CD80

  • Purified anti-mouse CD80

  • PE/Cyanine5 anti-mouse CD80

  • APC anti-mouse CD80

  • Alexa Fluor® 488 anti-mouse CD80

  • Alexa Fluor® 647 anti-mouse CD80

  • PerCP/Cyanine5.5 anti-mouse CD80

  • Pacific Blue™ anti-mouse CD80

  • Brilliant Violet 421™ anti-mouse CD80

  • Brilliant Violet 605™ anti-mouse CD80

  • Brilliant Violet 650™ anti-mouse CD80

  • PE/Cyanine7 anti-mouse CD80

  • Purified anti-mouse CD80 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD80

  • APC/Fire™ 750 anti-mouse CD80

  • Brilliant Violet 711™ anti-mouse CD80

  • Brilliant Violet 510™ anti-mouse CD80

  • TotalSeq™-A0849 anti-mouse CD80

  • TotalSeq™-C0849 anti-mouse CD80

  • Ultra-LEAF™ Purified anti-mouse CD80

  • Alexa Fluor® 594 anti-mouse CD80

  • TotalSeq™-B0849 anti-mouse CD80 Antibody

  • PE/Fire™ 640 anti-mouse CD80

  • Spark NIR™ 685 anti-mouse CD80
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account