Ultra-LEAF™ Purified anti-human CD178 (Fas-L) Antibody

Pricing & Availability
Clone
NOK-1 (See other available formats)
Workshop
VII 70322
Other Names
Fas Ligand (FasL), CD95L, TNFSF6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NOK-1_LEAF_071607
Human Fas Ligand transfected cells stained with LEAF™ purified NOK-1, followed by biotinylated anti-mouse IgG and Sav-PE
  • NOK-1_LEAF_071607
    Human Fas Ligand transfected cells stained with LEAF™ purified NOK-1, followed by biotinylated anti-mouse IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
306415 100 µg 160€
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306416 1 mg 536€
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Description

CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human FasL
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICC, IP, WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 306409).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
  2. Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
  3. Bossi G, et al. 1999. Nature Medicine 5:90.
  4. Andreola G, et al. 2002. J. Exp. Med. 195:1303.
  5. Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
  6. Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
  7. Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
  8. Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
  9. Qin G, et al. 2012. J. Infect. Dis. PubMed
  10. Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
  11. Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
  12. Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
  13. Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
  14. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
  15. Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed

Antigen Details

Structure
TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Distribution

Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells

Function
Apoptosis, immune privilege
Ligand/Receptor
CD95
Cell Type
Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.

Gene ID
356 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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