- NOK-1 (See other available formats)
- VII 70322
- Other Names
- Fas Ligand (FasL), CD95L, TNFSF6
- Mouse IgG1, κ
- Ave. Rating
- 3 reviews
- Product Citations
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CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.Product Details
- Human, Baboon
- Antibody Type
- Host Species
- L5178Y mouse T lymphoma cells expressing recombinant human FasL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 306409).
(PubMed link indicates BioLegend citation)
- Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
- Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
- Bossi G, et al. 1999. Nature Medicine 5:90.
- Andreola G, et al. 2002. J. Exp. Med. 195:1303.
- Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
- Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
- Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
- Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
- Qin G, et al. 2012. J. Infect. Dis. PubMed
- Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
- Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
- Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
- Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
- Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
- Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
- Product Citations
AB_314603 (BioLegend Cat. No. 306406)
AB_2100664 (BioLegend Cat. No. 306407)
- TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells
- Apoptosis, immune privilege
- Cell Type
- Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.
- Gene ID
- 356 View all products for this Gene ID
- View information about CD178 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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