- NOK-1 (See other available formats)
- VII 70322
- Other Names
- Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
- Mouse IgG1, κ
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- Product Citations
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CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.Product Details
- Human, Baboon
- Antibody Type
- Host Species
- L5178Y mouse T lymphoma cells expressing recombinant human FasL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306409).
(PubMed link indicates BioLegend citation)
- Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
- Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
- Bossi G, et al. 1999. Nature Medicine 5:90.
- Andreola G, et al. 2002. J. Exp. Med. 195:1303.
- Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
- Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
- Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
- Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
- Qin G, et al. 2012. J. Infect. Dis. PubMed
- Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
- Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
- Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
- Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
- Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
- Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
AB_2716104 (BioLegend Cat. No. 306411)
AB_2716105 (BioLegend Cat. No. 306412)
- TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells
- Apoptosis, immune privilege
- Cell Type
- Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.
- Gene ID
- 356 View all products for this Gene ID
- View information about CD178 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
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