Alexa Fluor® 700 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5slash114_Alx700_020608
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
  • M5slash114_Alx700_020608
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2)Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
107621 25 µg 85 CHF
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107622 100 µg 195 CHF
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
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  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
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  4. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
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  12. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
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  14. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  15. Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
  16. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
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  18. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  19. Mancini M, et al. 2019. J Immunol. 202:1479. PubMed
  20. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
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  24. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  25. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
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  28. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
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  45. Huang W, et al. 2015. J Allergy Clin Immunol. . PubMed
  46. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
  47. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  48. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  49. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  50. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  51. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  52. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  53. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  54. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
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  57. Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
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  59. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  60. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
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  62. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
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RRID
AB_493726 (BioLegend Cat. No. 107621)
AB_493727 (BioLegend Cat. No. 107622)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 10.30.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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