Purified anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5/114.15.2
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5/114.15.2
    C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5slash114_Pure_I-A-I-E_Antibody_102518
    Fresh, frozen mouse spleen was stained with purified I-A/I-E clone M5/114.15.2 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with F4/80 Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
107601 50 µg 55,00 CHF
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107602 500 µg 215,00 CHF
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  2. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  3. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  4. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  5. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  6. Zhang J, et al. 2019. J Clin Invest. 130:. PubMed
  7. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  8. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  9. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
  10. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  11. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  12. Hacini–Rachinel F, et al. 2018. J Allergy Clin Immunol. 141:163. PubMed
  13. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  14. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  15. Zaric M, et al. 2017. J Control Release. 268:166. PubMed
  16. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  17. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  18. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  19. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  20. Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed
  21. Heyman O, et al. 2018. Front Immunol. 1.563888889. PubMed
  22. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  23. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  24. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  25. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  26. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  27. Claser C, et al. 2019. Nat Commun. 3.361805556. PubMed
  28. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  29. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  30. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  31. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  32. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  33. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  34. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  35. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  36. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  37. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  38. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
  39. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  40. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  41. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  42. Sorrelle N, et al. 2019. J Immunol. 202:292. PubMed
  43. Sparber F, et al. 2018. PLoS Pathog. 14:e1007069. PubMed
  44. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  45. Luckashenak N, et al. 2006. J Immunol. 177:5177. PubMed
  46. Pascalis R, et al. 2008. Infect Immun. 76:4331. PubMed
  47. Matte-Martone C, et al. 2008. Blood. 111:3884. PubMed
  48. Kuns R, et al. 2009. Blood. 113:5999. PubMed
  49. Sabatino J, et al. 2011. J Exp Med. 208:81. PubMed
  50. Zhao J, et al. 2012. J Virol. 86:11416. PubMed
  51. Jux B, et al. 2013. Int Immunol. 25:307. PubMed
  52. König S, et al. 2014. PLoS One. 9:93555. PubMed
  53. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  54. Perez-Muñoz M, et al. 2015. PLoS One. 10: 0144382. PubMed
  55. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  56. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  57. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  58. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  59. Lau SF, et al. 2020. Cell Rep. 31:107530. PubMed
  60. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  61. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  62. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
RRID
AB_313316 (BioLegend Cat. No. 107601)
AB_313317 (BioLegend Cat. No. 107602)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 10.25.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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