Ultra-LEAF™ Purified anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OKT3_LEAF_021508
Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
  • OKT3_LEAF_021508
    Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
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317350 100 mg 3383 CHF
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317349 50 mg 2354 CHF
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317348 25 mg 1408 CHF
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317325 100 µg 99 CHF
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317326 1 mg 161 CHF
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317347 5 mg 583 CHF
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This product is eligible for a 40% discount! Promotion valid until December 31, 2023. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Zheng G, et al. 2021. Signal Transduct Target Ther. 6:236. PubMed
  2. Yoon HJ, et al. 2022. Exp Mol Med. Online ahead of print. PubMed
  3. Mancuso RV, et al. 2020. British Journal of Pharmacology. 177(12):2696-2711.. PubMed
  4. Babin L, et al. 2022. Mol Cancer. 21:65. PubMed
  5. Rieder SA, et al. 2020. Cell Mol Immunol. . PubMed
  6. Hsiue EH, et al. 2021. Science. 371:. PubMed
  7. Liu Y, et al. 2021. Immunol Cell Biol. 99:697. PubMed
  8. Yuan X, et al. 2022. J Extracell Vesicles. 11:e12235. PubMed
  9. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  10. Nicolas P, et al. 2022. J Exp Med. 219:. PubMed
  11. Yi K, et al. 2022. Front Immunol. 12:802795. PubMed
  12. Bak RO, et al. 2018. Nat Protoc. 13:358. PubMed
  13. Wang J, et al. 2022. Nat Commun. 13:6866. PubMed
  14. Natoli M, et al. 2022. J Immunother Cancer. 10:. PubMed
  15. Liu W, et al. 2021. J Biol Chem. 297:101102. PubMed
  16. Fang F, et al. 2022. JCI Insight. 7:. PubMed
  17. Schofield DJ, et al. 2021. MAbs. 13:1857100. PubMed
  18. Chen X, et al. 2021. Theranostics. 11:3392. PubMed
  19. Campos Carrascosa L, et al. 2020. J Immunother Cancer. 8:00. PubMed
  20. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
  21. Yan B, et al. 2021. Sci Immunol. 6: . PubMed
  22. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  23. Camviel N, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  25. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  26. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  27. Saragovi A, et al. 2020. Elife. 9:00. PubMed
  28. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  29. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  30. Kinloch NN, et al. 2021. Nat Commun. 12:165. PubMed
  31. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  32. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  33. Dahal S, et al. 2022. Retrovirology. 19:18. PubMed
  34. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  35. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  36. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  37. Pan W, et al. 2020. JCI Insight. 5:00. PubMed
  38. Sekine T, et al. 2020. Cell. 183:158. PubMed
  39. Sun T, et al. 2019. Haematologica. 105:661. PubMed
  40. Sharma V, et al. 2021. ACR Open Rheumatol. 3:277. PubMed
  41. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  42. Thakkar D, et al. 2022. J Immunother Cancer. 10:. PubMed
  43. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  44. Correnti CE, et al. 2020. Nat Struct Mol Biol. 27:342. PubMed
  45. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  46. Hurov K, et al. 2021. J Immunother Cancer. 9:. PubMed
  47. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  48. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  49. Jiang J, et al. 2021. Front Immunol. 12:785549. PubMed
  50. Hu BS, et al. 2020. Cancer Cell Int. 1.189583333. PubMed
  51. Wang J, et al. 2019. Sci Rep. 9:8445. PubMed
  52. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  53. Winheim E, et al. 2021. PLoS Pathog. 17:e1009742. PubMed
  54. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  55. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
  56. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  57. Gorchs L, et al. 2022. Cancers (Basel). 14:. PubMed
  58. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
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  60. de Haan L, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2749889 (BioLegend Cat. No. 317350)
AB_2749888 (BioLegend Cat. No. 317349)
AB_2571995 (BioLegend Cat. No. 317348)
AB_11147370 (BioLegend Cat. No. 317325)
AB_11150592 (BioLegend Cat. No. 317326)
AB_2571994 (BioLegend Cat. No. 317347)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 6    Revision Date: 07.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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