Alexa Fluor® 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Poly4064 (See other available formats)
Regulatory Status
Donkey Polyclonal Ig
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Product Citations
C57BL/6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 488 (top image).
  • Poly4064_AF488_IgG_ANtibody_FC_022014.jpg
    C57BL/6 splenocytes were stained with NK-1.1 Brilliant Violet 421™ and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) Alexa Fluor® 488 (top image).
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406416 100 µg $141
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This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
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  8. Song J,et al. 2017. Arch Virol.. 10.1007/s00705-017-3592-x. PubMed
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  10. McCullough LD, et al. 2021. J Clin Invest. 131:. PubMed
  11. Chi A, et al. 2022. Front Endocrinol (Lausanne). 13:871548. PubMed
  12. Singh A, et al. 2020. Mol Oncol. 1.901388889. PubMed
  13. Burns JC, et al. 2020. eLife. 9:00. PubMed
  14. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  15. Ahmed Ali A, et al. 2016. Sci Rep. 6:36650. PubMed
  16. Sung P, et al. 2014. J Virol. 88:9233. PubMed
  17. Singh KS, et al. 2021. Nature. 589:597. PubMed
  18. Nie Y, et al. 2021. Virol Sin. 36:692. PubMed
  19. Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
AB_2563203 (BioLegend Cat. No. 406416)

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Go To Top Version: 3    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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