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Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Isotype
Donkey Polyclonal Ig
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Poly4064_AF555_IgG_Antibody_IF_030515
HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
  • Poly4064_AF555_IgG_Antibody_IF_030515
    HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
See Alexa Fluor® 555 spectral data
Cat # Size Price Quantity Avail. Save
406412 100 µg $120.00
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Reactivity
Rabbit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 555.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.0-5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 580 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Application Notes

This _ conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

RRID
AB_2563181 (BioLegend Cat. No. 406412)

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Go To Top Version: 2    Revision Date: 02/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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