HRP Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Isotype
Donkey Polyclonal Ig
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Product Citations
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406401 500 µL $120
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

WB - Quality tested
ELISA, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
  2. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  3. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
  4. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  5. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  6. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  7. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  8. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  9. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  10. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  11. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  12. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  13. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  14. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  15. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  16. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  17. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  18. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  19. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
RRID
AB_2099368 (BioLegend Cat. No. 406401)

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Go To Top Version: 3    Revision Date: 05/20/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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