Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_BV421_IgG_Antibody_IF_012213
293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).
  • Poly4064_BV421_IgG_Antibody_IF_012213
    293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).
Compare all formats See Brilliant Violet 421™ spectral data
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406410 125 µL $204
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested usage of this reagent is a 1:20-1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Muto M, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016050496. PubMed
  2. Doehl JSP, et al. 2022. Front Immunol. 12:795554. PubMed
  3. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  4. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  5. Zhang Q, et al. 2019. PLoS Biol. 17:e3000330. PubMed
  6. Onodera T, et al. 2015. Sci Rep. 5:16801. PubMed
  7. Madge P, et al. 2016. Sci Rep. 6:36012. PubMed
  8. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  9. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  10. Cao W, et al. 2016. Nat Commun. 7:11687. PubMed
  11. Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed
  12. Tsao LC, et al. 2022. JCI Insight. . PubMed
  13. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  14. Vogel R, Erez A, Altan-Bonnet G 2016. Nat Commun. 7: 12428. PubMed
  15. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  16. Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
  17. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  18. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  19. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  20. Yamasaki T, et al. 2018. J Virol. 92:e01458. PubMed
  21. Lamsoul I, et al. 2012. PLoS One. 7:e43798. PubMed
  22. Gao X, et al. 2021. Nature. 589:591. PubMed
  23. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  24. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  25. Bohlen CJ et al. 2017. Neuron. 94(4):759-773 . PubMed
  26. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  27. Becker L, et al. 2013. PLoS One. 8:e59452. PubMed
  28. Liddelow S, et al. 2017. Nature. 541:481-487. PubMed
  29. Moysi E, et al. 2021. Front Immunol. 12:683396. PubMed
RRID
AB_10897810 (BioLegend Cat. No. 406410)

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 02/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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