DyLight™ 649 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Poly4064 (See other available formats)
Regulatory Status
Donkey Polyclonal Ig
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Product Citations
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406406 100 µg $106
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This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with DyLight™ 649 under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICC - Quality tested

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 649  has a maximum absorption of 655nm and a maximum emission of 670nm (similar to Alexa Fluor® 647 and APC).

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Grimm P, et al. 2012. J Biol Chem. 287:37673. PubMed
  2. Canny S, et al. 2014. MBio. 11:1033. PubMed
  3. Ranjbar S, et al. 2015. Cell Rep. 13: 874-83. PubMed
  4. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  5. Kivelio A, et al. 2015. Acta Biomater. 15:1. PubMed
  6. Lund T, et al. 2013. PLoS One. 8:e58945. PubMed
  7. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  8. Altitia A, et al. 2017. Carbohydr Res. 10.1016/j.carres.2017.03.005. PubMed
  9. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  10. Senger K, et al. 2017. Exp Hematol.. 10.1016/j.exphem.2017.07.005. PubMed
  11. Wu Y, et al. 2012. J Immunol. 188:2914. PubMed
  12. Login FH, et al. 2019. FASEB J. :fj201802068RR. PubMed
  13. Kumar S, et al. 2017. J Neuroendocrinol. 10.1111/jne.12466. PubMed
  14. Okabe M, et al. 2021. Am J Physiol Renal Physiol. 320:F922. PubMed
  15. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  16. Chierico L, et al. 2017. PLoS One. 12(2):e0171815. PubMed
  17. Osawa H, et al. 2016. Br J Cancer. 114:1251-1260. PubMed
  18. Durek P, et al. 2016. Immunity. 45:1148-1161. PubMed
  19. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  20. Schmid VK, et al. 2022. Haematologica. . PubMed
  21. Abel T, et al. 2013. Blood. 122:2030. PubMed
  22. Trinité B, et al. 2014. PLoS One. 9:110719. PubMed
AB_1575135 (BioLegend Cat. No. 406406)

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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