PE Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
  • Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
    C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
Compare all formats See PE spectral data
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406421 100 µg $104
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Ramovs V, et al. 2019. Breast Cancer Res. 21:63. PubMed
  2. Suzuki K, et al. 2019. Int J Mol Med. 44:1187. PubMed
  3. Komegae EN, et al. 2019. Front Immunol. 10:1496. PubMed
  4. Shukla GS, et al. 2020. Clin Transl Oncol. 22:1094. PubMed
  5. Kim EH, et al. 2020. Elife. 9:00. PubMed
  6. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  7. Hsiue EH, et al. 2021. Science. 371:. PubMed
  8. Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed
  9. Ramovs V, et al. 2020. J Invest Dermatol. . PubMed
  10. Liang F, et al. 2021. Front Immunol. 12:658715. PubMed
  11. Zuidema A, et al. 2022. J Cell Sci. 135:. PubMed
  12. Adler D, et al. 2019. iScience. 15:39. PubMed
  13. Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
  14. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  15. Ryniawec JM, et al. 2022. Diagnostics (Basel). 12:. PubMed
  16. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  17. Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed
  18. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  19. Kim S, et al. 2021. Elife. 10:. PubMed
  20. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  21. Routhu NK, et al. 2021. Immunity. 54(3):542-556.e9. PubMed
  22. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  23. Routhu NK, et al. 2021. Nat Commun. 12:3587. PubMed
  24. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  25. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  26. Carmona-Rivera C, et al. 2020. JCI Insight. 5:00. PubMed
  27. You C, et al. 2021. Cancers (Basel). 13:. PubMed
  28. Secchia S, et al. 2022. Dev Cell. 57:496. PubMed
  29. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  30. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  31. Thirugnanam K, et al. 2019. J Am Heart Assoc. 8:e012792. PubMed
  32. Zuidema A, et al. 2018. J Cell Sci. 131. PubMed
  33. Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed
  34. Fujiwara Y, et al. 2020. Clin Transl Immunology. 9:e1162. PubMed
  35. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  36. Claus M, Wingert S, Watzl C 2016. Open Bio. 6: 160010. PubMed
  37. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  38. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  39. Kwok HS, et al. 2019. ACS Chem Biol. 14:603. PubMed
  40. van Nie L, et al. 2020. Sci Rep. 10:11928. PubMed
RRID
AB_2563484 (BioLegend Cat. No. 406421)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/09/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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