- PC61 (See other available formats)
- Regulatory Status
- Other Names
- IL-2Rα, Ly-43, p55, Tac
- Rat IgG1, λ
- Ave. Rating
- Submit a Review
- Product Citations
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CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- IL-2-dependent cytolytic mouse T-cell clone B6.1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
- Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
- Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
- Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
- Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
- Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
- Lei TC, et al. 2005. Blood 105:4865. (Deplete)
- Pasare C, et al. 2004. Immunity 21:733. (Deplete)
- León-Ponte M, et al. 2007. Blood 109:3139.
- Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
- Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
- Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
- Product Citations
AB_893291 (BioLegend Cat. No. 102029)
AB_893288 (BioLegend Cat. No. 102030)
- Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
- IL-2 receptor
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
- Taniguchi T, et al. 1993. Cell 73:5-8.
- Waldmann TA. 1991. J Biol Chem. 266:2681-4.
- Read S, et al. 2000. J Exp Med. 192:295-302.
- Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
- Gene ID
- 16184 View all products for this Gene ID
- View information about CD25 on UniProt.org
Related Pages & Pathways
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other FormatsView All CD25 Reagents Request Custom Conjugation
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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