APC anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
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Product Citations
publications
pc61
Con A-stimulated (3 days)splenocytes stained with PC61 APC
  • pc61
    Con A-stimulated (3 days)splenocytes stained with PC61 APC
Compare all formats See APC spectral data
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102011 25 µg £37
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102012 100 µg £107
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Schuster M, et al. 2017. J Immunol. 199:920. PubMed
  2. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  3. Shi L, et al. 2019. Theranostics. 9:4115. PubMed
  4. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  5. Prajeeth C,et al. 2017. J Neuroinflammation.. 10.1186/s12974-017-0978-3. PubMed
  6. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  7. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  8. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  9. Noris M, et al. 2009. J Immunol. 183:4249. PubMed
  10. Kolbus D, et al. 2011. Immunobiology. 216:663. PubMed
  11. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  12. Annu K, et al. 2020. Sci Rep. 10:2359. PubMed
  13. Cheng G, et al. 2021. iScience. 24:102673. PubMed
  14. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  15. Waysbort N, et al. 2013. J Immunol. 191:5822. PubMed
  16. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  17. Kang TH, et al. 2019. Tissue Eng Regen Med. 16:539. PubMed
  18. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  19. Shi L, et al. 2021. Immunity. . PubMed
  20. Stahlhut M, et al. 2021. Sci Rep. 11:5294. PubMed
  21. Zimmermann J, et al. 2017. Eur J Immunol. 10.1002/eji.201747100. PubMed
  22. Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
  23. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  24. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  25. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  26. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  27. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  28. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  29. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  30. Wang SS, et al. 2021. Biomedicines. 9:. PubMed
  31. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  32. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  33. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  34. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  35. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  36. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  37. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  38. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  39. Glassman CR, et al. 2021. eLife. 10:00. PubMed
  40. Dou G, et al. 2020. Sci Adv. 6:eaba2987. PubMed
  41. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  42. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  43. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  44. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  45. Wigren M, et al. 2011. J Intern Med. 269:546. PubMed
  46. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  47. Liu X, et al. 2021. Int J Nanomedicine. 16:5675. PubMed
  48. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  49. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  50. Oomizu S, et al. 2012. Clin Immunol. 143:51. PubMed
  51. Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed
  52. Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed
  53. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  54. Kang CK, et al. 2021. J Infect Dis. 224:39. PubMed
  55. Tu J, et al. 2022. Mol Ther Nucleic Acids. 27:733. PubMed
  56. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  57. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  58. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  59. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  60. Ahn SH, et al. 2022. Front Endocrinol (Lausanne). 13:846226. PubMed
  61. Martomo SA, et al. 2021. Mol Cancer Ther. 1.074305556. PubMed
  62. Long C, et al. 2016. Toxicol Sci. 152: 85 - 98. PubMed
  63. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  64. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  65. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  66. Chen X, et al. 2019. Med Sci Monit. 25:5389. PubMed
  67. Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
  68. O'Donoghue GP, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  69. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  70. Hoover AR, et al. 2022. Clin Transl Med. 12:e937. PubMed
  71. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  72. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  73. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  74. Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
  75. Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed
  76. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  77. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  78. Tanaka S, et al. 2016. Appl Envir Microbiol. 82: 2693 - 2699. PubMed
  79. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  80. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  81. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  82. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  83. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  84. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  85. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  86. Deptuch T, et al. 2022. Sci Rep. 12:13480. PubMed
  87. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  88. Angelou CC, et al. 2020. Frontiers in Immunology. 2.586805556. PubMed
  89. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  90. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  91. Erens C, et al. 2022. Biomedicines. 10:. PubMed
  92. Hester AK, et al. 2022. Cell Rep. 38:110363. PubMed
  93. Park JS, et al. 2022. J Transl Med. 20:85. PubMed
  94. Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
  95. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  96. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
RRID
AB_312860 (BioLegend Cat. No. 102011)
AB_312861 (BioLegend Cat. No. 102012)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD25 PC61 FC
Biotin anti-mouse CD25 PC61 FC
FITC anti-mouse CD25 PC61 FC
PE anti-mouse CD25 PC61 FC
PE/Cyanine5 anti-mouse CD25 PC61 FC
Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
PE/Cyanine7 anti-mouse CD25 PC61 FC
Alexa Fluor® 488 anti-mouse CD25 PC61 FC
Alexa Fluor® 647 anti-mouse CD25 PC61 FC
Pacific Blue™ anti-mouse CD25 PC61 FC
Alexa Fluor® 700 anti-mouse CD25 PC61 FC
APC/Cyanine7 anti-mouse CD25 PC61 FC
PerCP/Cyanine5.5 anti-mouse CD25 PC61 FC
PerCP anti-mouse CD25 PC61 FC
Brilliant Violet 421™ anti-mouse CD25 PC61 FC
Brilliant Violet 605™ anti-mouse CD25 PC61 FC
Brilliant Violet 650™ anti-mouse CD25 PC61 FC
Ultra-LEAF™ Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Brilliant Violet 510™ anti-mouse CD25 PC61 FC
PE/Dazzle™ 594 anti-mouse CD25 PC61 FC
Brilliant Violet 711™ anti-mouse CD25 PC61 FC
Brilliant Violet 785™ anti-mouse CD25 PC61 FC
Alexa Fluor® 594 anti-mouse CD25 PC61 IHC-F
APC/Fire™ 750 anti-mouse CD25 PC61 FC
TotalSeq™-A0097 anti-mouse CD25 PC61 PG
KIRAVIA Blue 520™ anti-mouse CD25 PC61 FC
TotalSeq™-B0097 anti-mouse CD25 PC61 PG
TotalSeq™-C0097 anti-mouse CD25 PC61 PG
Spark NIR™ 685 anti-mouse CD25 Antibody PC61 FC
PE/Fire™ 640 anti-mouse CD25 PC61 FC
Spark YG™ 581 anti-mouse CD25 PC61 FC
APC/Fire™ 810 anti-mouse CD25 PC61 FC
Brilliant Violet 750™ anti-mouse CD25 PC61 FC
PerCP/Fire™ 780 anti-mouse CD25 PC61 FC
PE/Fire™ 700 anti-mouse CD25 PC61 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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