Purified anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
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Product Citations
publications
PC61_Pure_091907
C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC
  • PC61_Pure_091907
    C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC
  • PC61_pure_CD25_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified CD25 clone PC61 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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102002 500 µg £65.00
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block, Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  2. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  3. Murphy SF, et al. 2017. Pain. 158:1517. PubMed
  4. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  5. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  6. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  7. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  8. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  9. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  10. Kamio K, et al. 2018. Respir Res. 0.840972222. PubMed
  11. Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
  12. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  13. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  14. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  15. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  16. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  17. Liu F, et al. 2017. Front Immunol. 8:414. PubMed
  18. Ma F, et al. 2020. Theranostics. 5.784722222. PubMed
  19. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
  20. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  21. Gottschalk R, et al. 2012. Proc Natl Acad Sci U S A. 109:881. PubMed
  22. Anguela X, et al. 2013. Diabetes. 62:551. PubMed
  23. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  24. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  25. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  26. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
RRID
AB_312851 (BioLegend Cat. No. 102002)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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