Brilliant Violet 510™ anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5114.15.2_BV510_IAIE_Antibody_FC_091712
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
  • M5114.15.2_BV510_IAIE_Antibody_FC_091712
    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Avail. Save
107635 125 µL £109
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107636 50 µg £154
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
µg & µl: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
µg & µl: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/ml
µl sizes: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
µg & µl: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using µg size, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For flow cytometric staining using µl size, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Eddy W, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1601777. PubMed
  2. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  3. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  4. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  5. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  6. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  7. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  8. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  9. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  10. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  11. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  12. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  13. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  14. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  15. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  16. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  17. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  18. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  19. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  20. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  21. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  22. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  23. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  24. Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed
  25. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  26. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  27. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  28. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  29. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  30. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  31. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  32. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  33. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  34. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  35. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  36. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  37. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  38. Baasch S, et al. 2021. Cell. . PubMed
RRID
AB_2561397 (BioLegend Cat. No. 107635)
AB_2734168 (BioLegend Cat. No. 107636)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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