Alexa Fluor® 488 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5slash114_Alx488_011108
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
  • M5slash114_Alx488_011108
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
  • M5slash114-15-2_A488_I-A-I-E_Antibody_2_092121.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 488 spectral data
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107615 25 µg £58.00
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107616 100 µg £133.00
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  2. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  3. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  4. Xu D et al. 2018. Cell. 174(6):1477-1491 . PubMed
  5. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  6. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  7. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  8. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  9. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  10. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
  11. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  12. Napp J, et al. 2019. Theranostics. 4.754861111. PubMed
  13. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  14. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  15. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  16. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  17. Weilhammer D, et al. 2013. Biomaterials. 34:10305. PubMed
  18. Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
  19. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  20. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  21. OlguÍn-Alor R, et al. 2016. PLoS One. 11:e0167813. PubMed
  22. Yang H, et al. 2020. Arterioscler Thromb Vasc Biol. 40:e350. PubMed
  23. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  24. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  25. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  26. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  27. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  28. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  29. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  30. Korin B, et al. 2020. Sleep. :43. PubMed
  31. Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
RRID
AB_493524 (BioLegend Cat. No. 107615)
AB_493523 (BioLegend Cat. No. 107616)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 09/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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