Biotin anti-human CD138 Syndecan-1 Antibody, CD138, MI15

Pricing & Availability

Clone: MI15 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B-B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

MI15_Biotin_CD138_Antibody_021519 — Human myeloma cell line U266 was stained with CD138 (Syndecan-1) (clone MI15) Biotin (filled histogram) or mouse IgG1, κ Biotin isotype control (open histogram), followed by SAV-PE.

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356535

100 µg

£182

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

A mixture of U266 and XG-1 human myeloma cell lines.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138⁺ cell lines.⁴

Additional reported applications for the relevant formats include: immunofluorescent staining¹, Western blotting², immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections³, and spatial biology (IBEX)^5,6.

Application References (PubMed link indicates BioLegend citation)

Costes V, et al. 1999. Hum. Pathol. 30:1405. (IF)
Gattei V, et al. 1999. Br. J. Haematol. 104:152. (WB)
Bologna-Molina R, et al. 2008. Oral Oncol. 44:805. (IHC)
Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482.
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2800964 (BioLegend Cat. No. 356535)

Antigen Details

Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, controls cell morphology, regulates cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD138 (Syndecan-1)	MI15	FC,SB
Purified anti-human CD138 (Syndecan-1)	MI15	FC,ICC,WB,IHC-P
APC anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138 (Syndecan-1)	MI15	FC
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)	MI15	FC
PE/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)	MI15	FC,SB
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)	MI15	ICC
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)	MI15	FC
APC/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Pacific Blue™ anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)	MI15	PG
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)	MI15	FC
Biotin anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)	MI15	PG
APC/Fire™ 750 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Cyanine5 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Fire™ 640 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 500 anti-human CD138 (Syndecan-1)	MI15	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02/14/2019

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