- 29A1.4 (See other available formats)
- Regulatory Status
- Other Names
- NKp46, NCR1
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- NKP46-IgG1 Fc fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
- Application References
- Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
- Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
- Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
- Product Citations
AB_2563744 (BioLegend Cat. No. 137625)
- Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
- NK cells activation
- Viral hemagglutinins, heparan sulfate proteoglycans
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
- Gene ID
- 17086 View all products for this Gene ID
- View information about CD335 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Brilliant Violet 510™ anti-mouse CD335 (NKp46)
Brilliant Violet 711™ anti-mouse CD335 (NKp46)
PE anti-mouse CD335 (NKp46)
PE/Cyanine7 anti-mouse CD335 (NKp46)
Purified anti-mouse CD335 (NKp46)
FITC anti-mouse CD335 (NKp46)
APC anti-mouse CD335 (NKp46)
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)
Brilliant Violet 421™ anti-mouse CD335 (NKp46)
Biotin anti-mouse CD335 (NKp46)
Brilliant Violet 605™ anti-mouse CD335 (NKp46)
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)
Alexa Fluor® 647 anti-mouse CD335 (NKp46)
PE/Dazzle™ 594 anti-mouse CD335 (NKp46)
APC/Fire™ 750 anti-mouse CD335 (NKp46)
Brilliant Violet 650™ anti-mouse CD335 (NKp46)
TotalSeq™-A0184 anti-mouse CD335 (NKp46)
Brilliant Violet 785™ anti-mouse CD335 (NKp46)
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)
TotalSeq™-B0184 anti-mouse CD335 (NKp46)
TotalSeq™-C0184 anti-mouse CD335 (NKp46)
APC/Cyanine7 anti-mouse CD335 (NKp46)
PE/Cyanine5 anti-mouse CD335 (NKp46)
PerCP/Fire™ 806 anti-mouse CD335 (NKp46)
PerCP/Fire™ 780 anti-mouse CD335 (NKp46)