Purified anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
29A1.4 (See other available formats)
Regulatory Status
Other Names
NKp46, NCR1
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with purified 29A1.4 conjugated with PE and NK1.1 (PK136) APC
  • 29A1dot4_PE_030510
    C57BL/6 mouse splenocytes stained with purified 29A1.4 conjugated with PE and NK1.1 (PK136) APC
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137601 50 µg 81€
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137602 500 µg 246€
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CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
NKP46-IgG1 Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Guerriero J, et al. 2011. J Immunol. 186:3517. PubMed
  3. Wang C, et al. 2020. Oncoimmunology. 9:1809947. PubMed
  4. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  5. Puca E, et al. 2020. J Gastrointest Oncol. 1.015972222. PubMed
  6. Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
  7. Sodir NM, et al. 2022. Nat Commun. 13:6782. PubMed
  8. Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed
  9. Drzyzga A, et al. 2021. Cancers (Basel). 13: . PubMed
  10. Xiang H, et al. 2021. MAbs. 13:1981202. PubMed
  11. Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed
  12. Kortlever RM et al. 2017. Cell. 171(6):1301-1315 . PubMed
  13. Bajic D, et al. 2020. Mol Cancer Ther. 19:2554. PubMed
  14. Puca E, et al. 2020. Int J Cancer. 2518:146. PubMed
  15. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  16. Staaf E, et al. 2018. Sci Signal. 11:00. PubMed
  17. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  18. Aguilar OA, et al. 2020. J Immunol. 205:1709. PubMed
  19. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  20. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  21. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Crinier A, et al. 2018. Immunity. 49:971. PubMed
  23. Martin E, et al. 2020. JCI Insight. :5. PubMed
  24. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  25. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
AB_10551441 (BioLegend Cat. No. 137601)
AB_10552740 (BioLegend Cat. No. 137602)

Antigen Details

Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

NK cells activation
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
View information about CD335 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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