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APC/Fire™ 750 anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
29A1point4_APCFire750_CD335_Antibody_1_062316
C57BL/6 splenocytes were stained with NK1.1 PE and CD335 (clone 29A1.4) APC/Fire™ 750 (top) or rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).
  • 29A1point4_APCFire750_CD335_Antibody_1_062316
    C57BL/6 splenocytes were stained with NK1.1 PE and CD335 (clone 29A1.4) APC/Fire™ 750 (top) or rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).
  • 29A1point4_APCFire750_CD335_Antibody_2_062316
Compare all formats See APC/Fire™ 750 spectral data
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137631 25 µg 118€
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137632 100 µg 278€
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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Sweeney EE, et al. 2020. Nano Res. 13:736. PubMed
  2. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  3. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
RRID
AB_2617040 (BioLegend Cat. No. 137631)
AB_2617041 (BioLegend Cat. No. 137632)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • TotalSeq™-C0184 anti-mouse CD335 (NKp46)

  • APC/Cyanine7 anti-mouse CD335 (NKp46)

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