Brilliant Violet 711™ anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
29A1.4 (See other available formats)
Regulatory Status
Other Names
NKp46, NCR1
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
C57BL/6 splenocytes were stained with NK1.1 (clone PK136) APC and NKp46 (clone 29A1.4) Brilliant Violet 711™ (top) or rat IgG2a Brilliant Violet 711™ isotype control (bottom).
  • 29A1.4_BV711_CD335_Antibody_FC_1_042214
    C57BL/6 splenocytes were stained with NK1.1 (clone PK136) APC and NKp46 (clone 29A1.4) Brilliant Violet 711™ (top) or rat IgG2a Brilliant Violet 711™ isotype control (bottom).
  • 29A1.4_BV711_CD335_Antibody_FC_2_042214
Compare all formats See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Check Availability Save
137621 50 µg 248€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
NKP46-IgG1 Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Hofmann I, et al. 2022. J Pharmacol Exp Ther. Online ahead of print. PubMed
  2. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  3. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  4. Lu SX, et al. 2021. Cell. . PubMed
  5. Du X, et al. 2018. Proc Natl Acad Sci U S A. 115:E11731. PubMed
  6. Du W, et al. 2021. Mol Cancer Res. 19:1412. PubMed
  7. Crosse EI, et al. 2020. Cell Stem Cell. 27:822. PubMed
  8. Vicencio JM, et al. 2022. Cell Death Dis. 13:274. PubMed
  9. Goc J, et al. 2021. Cell. 184:5015. PubMed
  10. Dammeijer F, et al. 2020. Cancer Cell. 38(5):685-700.e8. PubMed
AB_2563289 (BioLegend Cat. No. 137621)

Antigen Details

Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

NK cells activation
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
View information about CD335 on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/22/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 510™ anti-mouse CD335 (NKp46)

  • Brilliant Violet 711™ anti-mouse CD335 (NKp46)

  • PE anti-mouse CD335 (NKp46)

  • PE/Cyanine7 anti-mouse CD335 (NKp46)

  • Purified anti-mouse CD335 (NKp46)

  • FITC anti-mouse CD335 (NKp46)

  • APC anti-mouse CD335 (NKp46)

  • PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)

  • Brilliant Violet 421™ anti-mouse CD335 (NKp46)

  • Biotin anti-mouse CD335 (NKp46)

  • Brilliant Violet 605™ anti-mouse CD335 (NKp46)

  • Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)

  • Alexa Fluor® 647 anti-mouse CD335 (NKp46)

  • PE/Dazzle™ 594 anti-mouse CD335 (NKp46)

  • APC/Fire™ 750 anti-mouse CD335 (NKp46)

  • Brilliant Violet 650™ anti-mouse CD335 (NKp46)

  • TotalSeq™-A0184 anti-mouse CD335 (NKp46)

  • Brilliant Violet 785™ anti-mouse CD335 (NKp46)

  • Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)

  • TotalSeq™-B0184 anti-mouse CD335 (NKp46)

  • TotalSeq™-C0184 anti-mouse CD335 (NKp46)

  • APC/Cyanine7 anti-mouse CD335 (NKp46)

  • PE/Cyanine5 anti-mouse CD335 (NKp46)

  • PerCP/Fire™ 806 anti-mouse CD335 (NKp46)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account