APC/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Other Names
Ly-76
Isotype
Rat IgG2b, κ
Ave. Rating
1 reviews
Product Citations
publications
TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
  • TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
    C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
116223 25 µg 85 CHF
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Xu X, et al. 2011. Am J Physiol Regul Integr Comp Physiol. 300:R1115. PubMed
  2. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
  3. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  4. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  5. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  6. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  7. McKenney AS, et al. 2018. J Clin Invest. 128:789. PubMed
  8. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  9. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  10. Xu R, et al. 2018. Nat Med. 24:823. PubMed
  11. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  12. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  13. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  14. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  15. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  16. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  17. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  18. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  19. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  20. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  21. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  22. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  23. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  24. Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
  25. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  26. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  27. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  28. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  29. Krautler NJ, et al. 2020. Cell Rep. 30:997. PubMed
RRID
AB_2137788 (BioLegend Cat. No. 116223)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02.01.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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