APC anti-mouse TER-119/Erythroid Cells Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)

1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
2. Vannucchi AM, et al. 2000. Blood 95:2559.
3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
4. Grisendi S, et al. 2005. Nature 437:147. (FC)
5. Bourdeau A, et al. 2007. Blood 109:4220.
6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
7. Heuser M, et al. 2007. Blood 110:1639. (FC)
8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

1. Koury D, et al. 2017. Int J Parasitol. 10.1016/j.ijpara.2017.05.009. PubMed
2. Wang L, et al. 2018. Bone Res. 6:22. PubMed
3. Yang D, et al. 2022. Cell. 185:1905. PubMed
4. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
5. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
6. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
7. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
8. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
9. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
10. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
11. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
12. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
13. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
14. Li C, et al. 2021. Mol Ther. 29:822. PubMed
15. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
16. Larouche JA, et al. 2021. eLife. 0.416666666666667. PubMed
17. Liu P, et al. 2020. Genome Res. 30:1655. PubMed
18. Bilodeau M, et al. 2009. PLoS One. 4:e7500. PubMed
19. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
20. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
21. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
22. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
23. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
24. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
25. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
26. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
27. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
28. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
29. Liu S, et al. 2021. Mol Ther. 29:1918. PubMed
30. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
31. Guihard S, et al. 2010. Blood. 115:3686. PubMed
32. Yu VWC, et al. 2017. Cell. 168:944. PubMed
33. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
34. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
35. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
36. Negretti NM, et al. 2021. Development. 148:. PubMed
37. Shcherbina A, et al. 2020. Cell Rep. 32:107964. PubMed
38. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
39. Saleiro D, et al. 2022. Nat Commun. 13:1750. PubMed
40. Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed
41. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
42. Crowe JL, et al. 2020. Proc Natl Acad Sci U S A. 22953:117. PubMed
43. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
44. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
45. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
46. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
47. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
48. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
49. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
50. Li G, et al. 2020. J Clin Invest. 130:1233. PubMed
51. Long JT, et al. 2022. Elife. 11:. PubMed
52. Wang H, et al. 2019. J Clin Invest. 129:598. PubMed
53. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
54. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
55. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
56. Song J, et al. 2014. Exp Cell Res. 327:123. PubMed
57. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
58. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
59. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
60. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
61. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
62. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
63. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
64. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
65. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
66. Maeda K, et al. 2021. Pathogens. 10:. PubMed
67. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
68. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
69. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
70. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
71. Wang H, et al. 2020. JCI Insight. 5:00. PubMed
72. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
73. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
74. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
75. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
76. Khoriaty R, et al. 2014. Mol Cell Biol. 34:3721. PubMed
77. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
78. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
79. Yin Y, et al. 2016. J Biol Chem. 291: 6923 - 6935. PubMed
80. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
81. Werner MT, et al. 2020. J Biol Chem. 295:1898. PubMed
82. Lam J, et al. 2018. Nat Commun. 9:2418. PubMed
83. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
84. Murray CW, et al. 2022. Nat Commun. 13:1090. PubMed

RRID

AB_313712 (BioLegend Cat. No. 116211)
AB_313713 (BioLegend Cat. No. 116212)

Antigen Details

Structure

Associated with glycophorin A, 52 kD

Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type

Erythrocytes

Biology Area

Immunology

Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID

104231 View all products for this Gene ID

UniProt

View information about TER-119 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis