APC/Cyanine7 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
  • 30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
    C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103115 25 µg 98 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103116 100 µg 196 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  3. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  4. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  5. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  6. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  7. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  8. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  9. Pattwell SS, et al. 2022. Sci Adv. 8:eabo6789. PubMed
  10. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  11. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  12. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  13. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  14. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  15. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  16. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  17. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  18. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  19. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  20. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  21. Freitag PC, et al. 2023. Mol Ther Methods Clin Dev. 29:120. PubMed
  22. Thai LM, et al. 2023. iScience. 26:106477. PubMed
  23. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  24. Sasaki L, et al. 2022. Nat Aging. 2:105. PubMed
  25. Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed
  26. Lee K, et al. 2023. JCI Insight. 8:. PubMed
  27. Wang C, et al. 2023. Int J Oral Sci. 15:19. PubMed
  28. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  29. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  30. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  31. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  32. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  33. Stefkovich M, et al. 2021. Mol Metab. 54:101357. PubMed
  34. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  35. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  36. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  37. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  38. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  39. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  40. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  41. Ren Y, et al. 2021. Am J Transplant. 21:552. PubMed
  42. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  43. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  44. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  45. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  46. He D, et al. 2022. Immunity. 55:159. PubMed
  47. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  48. Goncharov NV, et al. 2022. J Evol Biochem Physiol. 58:230. PubMed
  49. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  50. Denorme F, et al. 2022. J Clin Invest. 132: . PubMed
  51. Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed
  52. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  53. Miyao T, et al. 2022. Elife. 11: . PubMed
  54. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  55. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  56. Campisi L, et al. 2022. Nature. 606:945. PubMed
  57. Kenigsbuch M, et al. 2022. Nat Neurosci. 25:876. PubMed
  58. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  59. Li F, et al. 2022. Gut Microbes. 14:2096989. PubMed
  60. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  61. Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed
  62. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  63. Zhu X, et al. 2023. Adv Mater. 35:e2207198. PubMed
  64. Li S, et al. 2022. Nat Commun. 13:7371. PubMed
  65. Morrow RJ, et al. 2022. Cells. 11: . PubMed
  66. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  67. Alula KM, et al. 2023. Sci Rep. 13:18. PubMed
  68. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  69. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  70. Zhou J, et al. 2023. Nutrients. 15: . PubMed
  71. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  72. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  73. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  74. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  75. Chun J, et al. 2023. Cancers (Basel). 15: . PubMed
  76. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  77. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  78. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  79. Marsh SE, et al. 2022. Nat Neurosci. 25:306. PubMed
  80. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  81. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  82. Ren D, et al. 2022. STAR Protoc. 3:101217. PubMed
  83. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  84. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  85. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  86. Page N, et al. 2018. Immunity. 48:937. PubMed
  87. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  88. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  89. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  90. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  91. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  92. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  93. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  94. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  95. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  96. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  97. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  99. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  100. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  101. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  102. Ebneter A, et al. 2017. Transl Vis Sci Technol. 10.1167/tvst.6.2.10. PubMed
  103. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  104. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  105. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  106. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  107. Pluchino N, et al. 2020. J Cell Mol Med. 24:2464. PubMed
  108. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  109. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  110. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  111. Baram T, et al. 2021. Cells. 10:. PubMed
  112. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  113. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  114. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  115. Adrover JM, et al. 2022. JCI Insight. 7:. PubMed
  116. Steubing RD, et al. 2022. Brain Behav Immun Health. 24:100493. PubMed
  117. Lu H, et al. 2021. Autophagy. 17:2511. PubMed
  118. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  119. Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed
  120. Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
  121. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  122. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  123. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  124. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  125. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  126. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  127. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  128. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  129. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  130. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  131. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  132. Zhou X, et al. 2022. Front Pharmacol. 13:887263. PubMed
  133. Biswas S, et al. 2021. Nature. 591:464. PubMed
  134. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  135. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  136. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  137. Wiley CD, et al. 2019. JCI Insight. 4:e130056. PubMed
  138. Krasteva G, et al. 2011. Proc Natl Acad Sci U S A. 108:9478. PubMed
  139. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  140. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  141. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  142. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  143. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  144. Jung BG, et al. 2022. PLoS Pathog. 18:e1010454. PubMed
  145. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  146. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  147. Mantia-Smaldone G, et al. 2014. Gynecol Oncol. 133:584. PubMed
  148. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  149. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  150. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  151. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  152. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  153. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  154. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  155. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  156. Ning F, et al. 2019. J Exp Med. 216:1396. PubMed
  157. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  158. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  159. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  160. Hammond TR, et al. 2019. Immunity. 50:253. PubMed
  161. Schönfeld M, et al. 2021. Alcohol Clin Exp Res. 45:1980. PubMed
  162. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  163. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  164. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  165. Marangoni F, et al. 2021. Cell. . PubMed
  166. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
  167. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  168. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  169. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  170. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  171. Commerford CD et al. 2018. Cell reports. . PubMed
  172. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
  173. Yousef H, et al. 2018. Bio Protoc. 8:22. PubMed
  174. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  175. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  176. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  177. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  178. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  179. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  180. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  181. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  182. Uher O, et al. 2021. Cancers (Basel). 13:. PubMed
  183. Liu M, et al. 2020. Cell Rep. 32:107990. PubMed
  184. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  185. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  186. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  187. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  188. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  189. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  190. Soldevilla MM, et al. 2019. Mol Ther. 27:1878. PubMed
  191. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  192. Li Y, et al. 2019. Sci Rep. 9:6932. PubMed
  193. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  194. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  195. Gao H, et al. 2021. Cells. 10:. PubMed
  196. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  197. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  198. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  199. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  200. Zhang X, et al. 2020. Elife. 9:00. PubMed
  201. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  202. Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
  203. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  204. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  205. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  206. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  207. Ali M, et al. 2022. JCI Insight. 7:. PubMed
  208. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  209. Cho YK, et al. 2022. Nat Commun. 13:4084. PubMed
  210. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  211. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  212. Veremeyko T, et al. 2012. J Vis Exp. 65: 4097. PubMed
  213. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  214. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  215. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  216. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  217. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  218. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  219. Neuhöfer P, et al. 2021. Nature. 597:715. PubMed
  220. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  221. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  222. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  223. Selvaraj UM, et al. 2021. Brain Behav Immun. 95:502. PubMed
  224. Ying T, et al. 2021. Am J Physiol Endocrinol Metab. 320:E1148. PubMed
  225. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  226. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  227. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  228. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  229. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  230. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  231. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  232. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  233. Martina M, et al. 2014. J Vis Exp. 87: 51192. PubMed
  234. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  235. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  236. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  237. Yang Y, et al. 2022. Elife. 11:. PubMed
  238. Abramowski P, et al. 2014. J Neuroimmunol. 274:111. PubMed
  239. Hoeksema M, et al. 2015. J Immunol. 194:3909. PubMed
  240. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  241. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  242. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  243. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  244. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  245. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  246. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  247. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  248. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  249. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  250. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  251. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  252. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  253. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  254. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  255. Gierut J, et al. 2015. Stem Cell Res. 15: 165-171. PubMed
  256. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  257. Haka A, et al. 2012. PLoS One. 7:e44823. PubMed
  258. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  259. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  260. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  261. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  262. Michaelis KA, et al. 2019. Brain Behav Immun. 82:338. PubMed
  263. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  264. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  265. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  266. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  267. Yu W, et al. 2022. Nat Commun. 13:3544. PubMed
  268. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  269. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  270. Favuzzi E, et al. 2021. Cell. 184(15):4048-4063.e32. PubMed
  271. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  272. Draper D, et al. 2012. J Immunol. 188:5327. PubMed
  273. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  274. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  275. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  276. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  277. Selvaraj UM et al. 2018. Endocrinology. 159(11):3848-3859 . PubMed
  278. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  279. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  280. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  281. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  282. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  283. Wang X, et al. 2022. Front Immunol. 13:864984. PubMed
  284. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  285. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  286. Ou YC, et al. 2020. ACS Nano. 1.035416667. PubMed
  287. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  288. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  289. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  290. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  291. Stock M, et al. 2015. Osteoarthritis Cartilage. 23: 1221-1225. PubMed
  292. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  293. Kaiser T, et al. 2019. Eneuro. 6. PubMed
  294. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  295. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  296. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  297. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  298. Lu Z, et al. 2021. J Clin Invest. 131:. PubMed
  299. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  300. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  301. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  302. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  303. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  304. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  305. Jiang P, et al. 2018. Nat Med. 24:1550. PubMed
  306. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  307. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  308. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  309. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  310. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  311. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  312. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  313. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
  314. Kline JM, et al. 2021. J Biol Chem. :100373. PubMed
  315. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  316. Starikova E, et al. 2021. Front Pharmacol. 12:667572. PubMed
  317. Klein S, et al. 2016. J Cell Sci. 129: 2573 - 2585. PubMed
  318. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  319. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  320. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  321. Martin A, et al. 2018. Oncotarget. 9:28364. PubMed
  322. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  323. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  324. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  325. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  326. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  327. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  328. Morein D, et al. 2021. Cells. 10: . PubMed
  329. Yan C, et al. 2022. NPJ Precis Oncol. 6:6. PubMed
  330. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  331. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  332. Yoldi G, et al. 2016. Cancer Res . 76: 5857 - 5869. PubMed
  333. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  334. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  335. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  336. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  337. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  338. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  339. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  340. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  341. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  342. Wang Y, et al. 2022. Nat Commun. 13:1454. PubMed
  343. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  344. Zhang X, et al. 2022. J Exp Clin Cancer Res. 41:246. PubMed
  345. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  346. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  347. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  348. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  349. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  350. Liu SS, et al. 2021. Immunity. :. PubMed
  351. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  352. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  353. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  354. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  355. Seeling M, et al. 2013. Proc Natl Acad Sci U S A. 110:10729. PubMed
  356. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  357. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  358. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  359. Forte E, et al. 2020. Cell Rep. 30:3149. PubMed
  360. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
  361. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  362. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  363. De Micheli AJ, et al. 2020. Am J Physiol Cell Physiol. 319:C885. PubMed
  364. Sibler E, et al. 2021. Cells. 10:. PubMed
  365. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
  366. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  367. Sebastian A, et al. 2021. Cells. 10:. PubMed
  368. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  369. Lux A, et al. 2014. Cell Rep. 10:236. PubMed
  370. Madsen P, et al. 2016. J Neurosci. 36: 5128 - 5143. PubMed
  371. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  372. Parker K, Horn L, Ostrand-Rosenberg S 2016. J Leukoc Biol. 100: 463 - 470.. PubMed
  373. Chen MB, et al. 2020. Cell Rep. 30:4418. PubMed
  374. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  375. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  376. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  377. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  378. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  379. Swanson WB, et al. 2021. Biomaterials. 272:120769. PubMed
  380. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  381. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  382. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  383. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  384. Parent AV, et al. 2021. Cell Reports. 36(7):109538. PubMed
  385. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  386. Ma Y, et al. 2021. Developmental Cell. 56(4):478-493.e11. PubMed
  387. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  388. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  389. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
RRID
AB_312980 (BioLegend Cat. No. 103115)
AB_312981 (BioLegend Cat. No. 103116)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV™ 395 anti-mouse CD45 30-F11 FC
Go To Top Version: 3    Revision Date: 01.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account