Biotin anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_Biotin_TER-119_Antibody_FC_041713
C57BL/6 bone marrow cells were stained with biotinylated TER-119 (filled histogram) or biotinylated rat IgG2b, κ isotype control (open histogram) and then detected with Sav-PE.
  • TER-119_Biotin_TER-119_Antibody_FC_041713
    C57BL/6 bone marrow cells were stained with biotinylated TER-119 (filled histogram) or biotinylated rat IgG2b, κ isotype control (open histogram) and then detected with Sav-PE.
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116203 50 µg 40 CHF
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116204 500 µg 120 CHF
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Peraki I, Palis J, and Mavrothalassitis G. 2017. Mol Cell Biol. 10.1128/MCB.00183-17. PubMed
  2. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
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  4. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
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  22. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
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  28. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
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  35. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  36. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  37. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  38. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
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  47. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
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  49. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  50. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
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  54. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
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  57. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  58. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
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  60. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
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  64. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
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  66. Verena Sigl et al. 2017. Cell research. 26(7):761-74 . PubMed
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RRID
AB_313704 (BioLegend Cat. No. 116203)
AB_313705 (BioLegend Cat. No. 116204)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 12.04.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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