PerCP/Cyanine5.5 anti-mouse CD95 (Fas) Antibody

Pricing & Availability
Clone
SA367H8 (See other available formats)
Regulatory Status
RUO
Other Names
Fas, ALPS1A, APO-1, APT1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SA367H8_PerCPCyanine5dot5_CD95_Antibody_020819_updated.png
C57BL/6 mouse splenocytes were stained with CD3 APC and with CD95 (clone SA367H8) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • SA367H8_PerCPCyanine5dot5_CD95_Antibody_020819_updated.png
    C57BL/6 mouse splenocytes were stained with CD3 APC and with CD95 (clone SA367H8) PerCP/Cyanine5.5 (left) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
152609 25 µg $110.00
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152610 100 µg $285.00
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Description

CD95, also known as Fas, is an approximately 45 kD type I transmembrane protein belonging to the TNFR superfamily (TNFRSF6). The expression of CD95 has been described in various organs, such as  thymus, spleen, liver, heart, lung and ovary. Upon ligand (FasL) binding, CD95 forms the death-inducing signaling complex (DISC) intracellularly and induce apoptosis of the cell. CD95-induced apoptosis plays important roles in development, as well as in maintining peripheral tolerance of the immune system.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Fas Transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Product Citations
  1. Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
  2. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  3. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
RRID
AB_2632904 (BioLegend Cat. No. 152609)
AB_2632905 (BioLegend Cat. No. 152610)

Antigen Details

Structure
CD95 (Fas) is an approximately 45 kD type I transmembrane protein; cystiene-rich domain, death domain.
Distribution

Thymus, spleen, liver, heart, lung, and ovary.

Function
Induction of apoptosis.
Interaction
Caspase 8, caspase 10, CFLAR, FADD, PDCD6, SUMO1, and TNFRSF6.
Ligand/Receptor
FasL.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Ogasawara J, et al. 1995. J. Exp. Med. 181:485.
2. Ogasawara J, et al. 1993. Nature. 364:806.
3. Nishimura Y, et al. 1995. J. Immunol. 154:4395.

Gene ID
14102 View all products for this Gene ID
UniProt
View information about CD95 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 02/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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