APC anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_APC_073012
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
  • 17A2_APC_073012
    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
100235 25 µg $30.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100236 100 µg $70.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  2. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  3. Zhang J, et al. 2018. Nature. 553:91. PubMed
  4. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  5. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  6. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  7. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  8. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  9. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  10. Zhang W, et al. 2019. Oncol Lett. 17:815. PubMed
  11. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  12. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  13. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
  14. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  15. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  16. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  17. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  18. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  19. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  20. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  21. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  22. Thomas S, et al. 2019. J Immunother Cancer. 7:214. PubMed
  23. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  24. Liu J, et al. 2019. Immunity. 50:600. PubMed
  25. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  26. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  27. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  28. Sartorius R, et al. 2018. Front Immunol. 1.413888889. PubMed
  29. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  30. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  31. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  32. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  33. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  34. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  35. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  36. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  37. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  38. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  39. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  40. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  41. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  42. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  43. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  44. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  45. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  46. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  47. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  48. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  49. Gardenier J, et al. 2017. Nat Commun. 8:14345. PubMed
  50. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  51. Li K, et al. 2020. Nat Commun. 4.844444444. PubMed
  52. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  53. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  54. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  55. Johnson DC, et al. 2020. Cell Death Dis. 0.894444444. PubMed
  56. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  57. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  58. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  59. Naito Y, et al. 2019. American Journal of Physiology-Renal Physiology. 318(1):F238-F247. PubMed
  60. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  61. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  62. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  63. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  64. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  65. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  66. Yu M, et al. 2021. Molecular Cell. 81(6):1216-1230.e9. PubMed
  67. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  68. Peng Z, et al. 2021. STAR Protocols. 2(2):100595. PubMed
RRID
AB_2561455 (BioLegend Cat. No. 100235)
AB_2561456 (BioLegend Cat. No. 100236)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Customers Also Purchased

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account