APC anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
3 reviews
Product Citations
publications
17A2_APC_073012
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
  • 17A2_APC_073012
    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
100235 25 µg $30
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100236 100 µg $70
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  4. Gardenier J, et al. 2017. Nat Commun. 8:14345. PubMed
  5. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  6. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  7. Zhang J, et al. 2018. Nature. 553:91. PubMed
  8. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  9. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  10. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  11. Zhang W, et al. 2019. Oncol Lett. 17:815. PubMed
  12. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  13. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  14. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
  15. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  16. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  17. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  18. Hou X, et al. 2019. Cell Rep. 28:172. PubMed
  19. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  20. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  21. Thomas S, et al. 2019. J Immunother Cancer. 7:214. PubMed
  22. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  23. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  24. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  25. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  26. Fanghui Zhang et al. 2019. Immunity. 50(3):738-750 . PubMed
  27. Liu J et al. 2019. Immunity. 50(3):600-615 . PubMed
  28. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  29. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  30. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  31. Sartorius R, et al. 2018. Front Immunol. 1.413888889. PubMed
  32. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  33. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  34. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  35. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  36. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
RRID
AB_2561455 (BioLegend Cat. No. 100235)
AB_2561456 (BioLegend Cat. No. 100236)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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