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Biotin anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
1_17A2_Biotin_CD3_Antibody_FC_1_072514
C57BL/6 splenocytes were stained with CD19 APC and biotinylated CD3 (clone 17A2) (top) or biotinylated rat IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • 1_17A2_Biotin_CD3_Antibody_FC_1_072514
    C57BL/6 splenocytes were stained with CD19 APC and biotinylated CD3 (clone 17A2) (top) or biotinylated rat IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • 2_17A2_Biotin_CD3_Antibody_FC_2_072514
  • 3_17A2_BIOTIN_CD3_Antibody_3_010620
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of biotin anti-mouse CD3 (clone 17A2), Alexa Fluor® 594 anti-mouse B220 (clone RA3-6B2) (blue) and Alexa Fluor® 488 anti-mouse CD29 (clone HMβ1-1) (green) overnight at 4°C, followed by 2.5 µg/ml of Spark YG™ 570 Streptavidin (red) for 2 hours at room temperature. The image was captured by 10X objective.
Cat # Size Price Quantity Avail. Save
100243 50 µg $40
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100244 500 µg $140
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Validated
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
Product Citations
  1. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  2. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  3. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  4. Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed
  5. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  6. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  7. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  8. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  9. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  10. Blecher‐Gonen R et al. 2019. Cell systems. 8(2):109-121 . PubMed
  11. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  12. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  13. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  14. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  15. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  16. Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed
RRID
AB_2563946 (BioLegend Cat. No. 100243)
AB_2563947 (BioLegend Cat. No. 100244)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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