PerCP/Cyanine5.5 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
6 reviews
Product Citations
publications
17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
  • 17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
100217 25 µg $100
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100218 100 µg $275
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
Product Citations
  1. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  2. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  3. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  4. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  5. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  6. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  7. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  8. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  9. Vidy A, et al. 2016. PLoS One. 11:e0165361. PubMed
  10. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  11. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  12. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  13. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  14. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  15. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  16. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  17. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  18. Soutto M, et al. 2017. J Cancer. 8:2424. PubMed
  19. Bennett FC et al. 2018. Neuron. 98(6):1170-1183 . PubMed
  20. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  21. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  22. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  23. Agarwal P et al. 2019. Cell stem cell. 24(5):769-784 . PubMed
  24. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  25. Li SX, et al. 2019. PLoS Pathog. 15:e1007611. PubMed
  26. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
RRID
AB_1595597 (BioLegend Cat. No. 100217)
AB_1595492 (BioLegend Cat. No. 100218)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 02/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Forgot your password? Reset Password
Request an Account