APC/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_APCCyanine7_CD3_Antibody_031919_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • 17A2_APCCyanine7_CD3_Antibody_031919_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100221 25 µg $105
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100222 100 µg $242
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mackel JJ, et al. 2022. Front Cell Infect Microbiol. 12:974175. PubMed
  2. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  3. Sun L, et al. 2021. Cancer Cell. :. PubMed
  4. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  5. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  7. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  8. Israelow B, et al. 2020. bioRxiv. . PubMed
  9. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  10. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  11. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  12. Chen S, et al. 2022. Cancer Discov. :. PubMed
  13. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  14. Durgeau A, et al. 2018. Nat Commun. 9:5097. PubMed
  15. Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed
  16. Diao L, et al. 2022. iScience. 25:105511. PubMed
  17. Tirado-Gonzalez I, et al. 2021. Cancer Discov. 11:2924. PubMed
  18. Herman JD, et al. 2021. Nat Commun. 12:6853. PubMed
  19. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  20. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  21. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  22. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  23. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  24. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  25. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  26. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  27. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  28. Chung H, et al. 2021. Immune Netw. 21:e28. PubMed
  29. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  30. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  31. Shi L, et al. 2021. Immunity. . PubMed
  32. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  33. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  34. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  35. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  36. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  37. Han JP, et al. 2022. Sci Adv. 8:eabj6901. PubMed
  38. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  39. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  40. Katsinas N, et al. 2022. J Clin Med. 11:. PubMed
  41. Xue L, et al. 2021. BMC Cancer. 21:1134. PubMed
  42. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  43. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  44. Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed
  45. Socodato R, et al. 2020. Sci Signal. 13: . PubMed
  46. Liu X, et al. 2022. Nat Cancer. :. PubMed
  47. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  48. Adane B, et al. 2019. Cell Rep. 27:238. PubMed
  49. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  50. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  51. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  52. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  53. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  54. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  55. Lang V, et al. 2021. Elife. 10:. PubMed
  56. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  57. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  58. Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  59. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  60. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  61. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  62. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  63. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  64. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  65. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  66. Revathikumar P, et al. 2018. PLoS One. 13:e0193210. PubMed
  67. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  68. Do-Thi VA, et al. 2021. Cancers (Basel). 13:. PubMed
  69. Teater M, et al. 2018. Nat Commun. 9:222. PubMed
  70. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  71. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  72. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  73. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  74. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  75. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  76. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  77. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  78. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  79. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  80. Rouse MD, et al. 2020. Front Microbiol. 11:414. PubMed
  81. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  82. Dokoshi T, et al. 2020. Cell Rep. 30:61. PubMed
  83. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  84. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  85. Li C, et al. 2021. Chem Biol Drug Des. 97:1151. PubMed
  86. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  87. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  88. Sharma R, et al. 2021. J Neuroinflammation. 72:18. PubMed
  89. Siolas D, et al. 2021. Cell Reports. 36(8):109578. PubMed
  90. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  91. Wen J, et al. 2015. J Pharmacol Sci. 128: 116-124. PubMed
  92. Wang K, et al. 2021. Nat Commun. 12:4558. PubMed
  93. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  94. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  95. Nakazawa S, et al. 2020. Sci Rep. 10:14559. PubMed
RRID
AB_2057374 (BioLegend Cat. No. 100221)
AB_2242784 (BioLegend Cat. No. 100222)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC, ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F, FC, 3D IHC, SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 03/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

  • Spark Red™ 718 anti-mouse CD3

ProductsHere

Login/Register
Remember me
Forgot your password? Reset Password
Request an Account