ELISA MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Other Names
TNF-a ELISA Kit
Ave. Rating
5 reviews
Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Save
430904 5 plates $260
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number, but will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  2. Cobb C 2010. J Immunol. 184:3789. PubMed
  3. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  4. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  5. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  6. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  7. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  8. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  10. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  11. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  12. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  13. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  14. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  15. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  16. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  17. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  18. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  19. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  20. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  21. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  22. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  23. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  24. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  25. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  26. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  27. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
Publication Library
Sensitivity
4 pg/ml
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe sets no longer come with plates, but Coating Buffer and Assay Diluent (for blocking and dilutions) are included in the ELISA MAX™ Deluxe Sets. Plates can be ordered separately (Cat. No. 423501), and instructions on coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 05/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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