ELISA MAX™ Deluxe Set Mouse IL-1β

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Regulatory Status
RUO
Other Names
IL-1b ELISA kit
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Product Citations
publications
mouse il-1b_122109
  • mouse il-1b_122109
Cat # Size Price Quantity Avail. Save
432604 5 plates $315
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432615 10 Plates $500
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432616 20 Plates $800
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-1β Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

BioLegend's mouse and human IL-1ß ELISA kits use antibodies raised against the mature form of IL-1ß. However, because pro-IL-1ß contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1ß ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1ß.

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Huck O, et al. 2014. Innate Immun. 21:65. PubMed
  3. Alves BN, et al. 2014. J. Immunol. 192:3121. PubMed
  4. Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
Product Citations
  1. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  2. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  3. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  4. Swaroop S, et al. 2018. J Neuroinflammation. 15:3385. PubMed
  5. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  6. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  7. Piedra-Quintero ZL, et al. 2019. Front Immunol. 9:3118. PubMed
  8. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  9. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  10. Huck O, et al. 2014. Innate Immunity. 21:65. PubMed
  11. Alves B, et al. 2014. J Immunol. 192:3121. PubMed
  12. Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
  13. Zeis T, et al. 2015. Brain Behav Immun. 48: 313-325. PubMed
  14. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  15. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  16. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  17. Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed
Sensitivity
16 pg/mL
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
16176 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 5    Revision Date: 11/16/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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