Direct-Blot™ HRP anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Other Names
Oncogene Myc, Myc proto-oncogene protein, proto-oncogene c-Myc
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
  • 9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
    Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
626804 25 µL $85
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626803 100 µL $245
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical data sheet

Product Details

Reactivity
Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
RRID
AB_2783361 (BioLegend Cat. No. 626804)
AB_2572009 (BioLegend Cat. No. 626803)

Antigen Details

Structure
Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution

Nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.

Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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