Anti-c-Myc Tag (9E10) Affinity Gel

Pricing & Availability
Clone
9E10 (See other available formats)
Other Names
Myc proto-oncogene protein, proto-oncogene c-Myc, transcription factor p64, class E basic helix-loop-helix protein 39, avian myelocytomatosis viral oncogene homolog, myc-related translation/localization
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
9E10_c-Myc_Tag_Gel_Purification_121213
Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_121213
    Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_2_121213
    Stable activity of anti-c-Myc agarose over ten rounds of affinity purification.
View Higher Resolution Data...
Cat # Size Price Quantity Avail. Save
658502 500 µl $175
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
50% anti-c-Myc-tag (9E10) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
Preparation
Affinity purified.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

Purification of c-Myc tagged fusion protein from bacteria lysates - Quality tested
IP - Reported in the literature

Recommended Usage

Resin binding capacity is greater than 0.5 mg/mL.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Shamay-Ramot A, et al. 2015. PLoS Genet. 11: 1005702. PubMed
  2. Zhang G, et al. 2016. J Virol. 90: 2306 - 2315. PubMed
Publication Library

Antigen Details

Structure
Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution

Nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Cell Targets
N-terminal, C-terminal tagged and internal fusion protein
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.

Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account