Anti-c-Myc Tag (9E10) Affinity Gel

Pricing & Availability
Clone
9E10 (See other available formats)
Other Names
Oncogene Myc, Myc proto-oncogene protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E10_c-Myc_Tag_Gel_Purification_121213
Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_121213
    Binding capacity of anti-c-Myc agarose for C-terminal c-Myc and internal c-Myc tagged GST.
  • 9E10_c-Myc_Tag_Gel_Purification_2_121213
    Stable activity of anti-c-Myc agarose over ten rounds of affinity purification.
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658502 500 µl $175
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Description

The c-myc protein is a 62 kD nuclear factor that is ubiquitously expressed in the nucleus. c-myc is part of a heterodimeric complex with MAX that acts as a potent transcriptional activator. c-myc is modified by glycosylation and phosphorylation and has been shown to interact with a number of proteins including SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, and Sp1. c-myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that the observed protein size is approximately 41 kD. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The 9E10 antibody has been shown to be useful in a number of applications including Western blotting, direct ELISA, flow cytometry, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and immunoaffinity purification of proteins expressing the human c-myc tag.

Product Details
Technical data sheet

Product Details

Reactivity
Human and fusion protein (EQKLISEEDL tag epitope) in all species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
50% anti-c-Myc-tag (9E10) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
Preparation
Affinity purified.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application

Purification of c-Myc tagged fusion protein from bacteria lysates - Quality tested
IP - Reported in the literature

Recommended Usage

Resin binding capacity is greater than 0.5 mg/mL.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry5 of formalin-fixed, paraffin-embedded tissue sections, ELISA1, immunofluorescence microscopy3,4, immunoprecipitation4, and immunoaffinity of c-myc-tagged fusion proteins6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schouten A, et al. 2002. J. Biol. Chem. 277:19339. (ELISA)
  2. Maher SE, et al. 1998. Transplantation 66:1094.
  3. Raftopoulou M, et al. 2004. Science 303:1179. (IF)
  4. Fan H, et al. 1998. Biochem. Cell. Biol. 76:125. (IF, IP)
  5. Korkolopoulou P, et al. 1994. Leuk Lymphoma 13:151. (IHC)
  6. Hillman MC, et al. 2001. Protein Expr. Purif. 23:359.
  7. Kondo, S., et al. 2011. J. Virol. 85:11255. PubMed
  8. Decarpentrie F, et al. 2012. Hum Mol Genet. PubMed
  9. Bourgeois-Daigneault MC, et al. 2012. J Immunol. 188:4959. PubMed
  10. Peters BM, et al. 2012. Microbiology. 158:2975. PubMed
  11. Robu M, et al. 2013. PNAS. 110:1658. PubMed
  12. Eilert E, et al. 2013. J Biotechnol. 167:85. PubMed
  13. Bi J, et al. 2013. Am J Physiol Renal Physiol. 305:532. PubMed
  14. Poe JC, et al. 2013. J exp Med. 511:57. PubMed
  15. Shkoporov AN, et al. 2015. FEMS Microbial Lett. 362:83. PubMed
Product Citations
  1. Shamay-Ramot A, et al. 2015. PLoS Genet. 11: 1005702. PubMed
  2. Zhang G, et al. 2016. J Virol. 90: 2306 - 2315. PubMed
Publication Library

Antigen Details

Structure
Highly conserved protein structure Drosophila to vertebrates. Contains HLH domain, coiled coil region, and leucine zipper domain, molecular weight approximately 62 kD
Distribution

Ubiquitously expressed nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Cell Targets
N-terminal, C-terminal tagged and internal fusion protein
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.

Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 11/08/2016

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